FastQCFastQC Report
Sat 3 Sep 2022
EGAF00004968434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004968434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1042903
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT198041.8989301977269217No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT183841.7627718014043492No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT158381.518645549969652No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70000.6712033621535272No Hit
CTTATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTC51060.48959490959370144No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT35500.3403959908064317No Hit
GTACATGGGTTTATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAA33550.32169818286072627No Hit
TCACAAAGACATCGACGCATTTACCGAACAACTCCGCGACATTTTTGTAGG33220.31853393843914535No Hit
ATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAAATGCGTCGATGT32020.30702759508794203No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT31700.30395923686095444No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28630.27452217512079263No Hit
TCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTGCTTGA28450.2727962236181122TruSeq Adapter, Index 10 (96% over 28bp)
ATCAATAACCTACAAAAATGTCGCGCGCATCACAAAGACATCGACGCATTT23330.22370249198631129No Hit
CATCACAAAGACATCGACGCATTTACCGAACAACTCCGCGACATTTTTGTA19350.1855397865381536No Hit
CTACAACACCGTCCTCCCACGTGCCCGGGTCCGTGTTGCTTGGTCTTCGTC18670.17901952530580506No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT14260.13673371349013283No Hit
ATACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTC14070.134911875792859TruSeq Adapter, Index 10 (95% over 22bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13020.12484382536055605No Hit
ACGCAGAGTACATGGGTTTATCAATAACCTACAAAAATGTCGCGGAGTTGT12260.11755647457146062No Hit
ATCTACAACACCGTCCTCCCACGTGCCCGGGTCCGTGTTGCTTGGTCTTCG11700.1121868476742324No Hit
ACACATCTCCGAGCCCACGAGACCGAGGCTGATCTCGTATGCCGTCTTCTG11200.10739253794456437RNA PCR Primer, Index 10 (95% over 24bp)
GTTTATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAAATGCGTCG10860.10413240732839008No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)