FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004968440

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004968440
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences826422
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT181862.2005706527657782No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT129491.5668750347885219No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT126981.5365031424623257No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT58330.7058137368075874No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23890.2890774930967472No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21890.26487678207985754No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT21510.2602786469866485No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17910.21671736715624706No Hit
ATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAAATGCGTCGATGT17820.215628335160487No Hit
TCACAAAGACATCGACGCATTTACCGAACAACTCCGCGACATTTTTGTAGG17810.21550733160540259No Hit
GTACATGGGTTTATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAA16440.19892984455883314No Hit
ATCAATAACCTACAAAAATGTCGCGCGCATCACAAAGACATCGACGCATTT15830.19154862769868178No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT15580.18852353882157058No Hit
CATCACAAAGACATCGACGCATTTACCGAACAACTCCGCGACATTTTTGTA12970.15694161094452955No Hit
ACGCAGAGTACATGGGTTTATCAATAACCTACAAAAATGTCGCGGAGTTGT9750.11797846620733718No Hit
GTTTATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAAATGCGTCG8810.10660413202939903No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8680.1050310858133012Clontech SMART CDS Primer II A (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)