FastQCFastQC Report
Sun 4 Sep 2022
EGAF00004968452

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004968452
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences1922155
Sequences flagged as poor quality0
Sequence length51
%GC42

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT396182.061124102894928No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT366841.9084829267150671No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT322111.675775366710801No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138940.7228345268721825No Hit
CTTATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTC99880.519625108276908No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT62460.32494777996571556No Hit
GTACATGGGTTTATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAA62220.32369918138755716No Hit
TCACAAAGACATCGACGCATTTACCGAACAACTCCGCGACATTTTTGTAGG61010.31740416355600876No Hit
ATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAAATGCGTCGATGT59940.3118374948950527No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT56980.29643811243109947No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT54330.28265150313060083No Hit
TCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTTGA53570.2786976076330993RNA PCR Primer, Index 33 (96% over 29bp)
ATCAATAACCTACAAAAATGTCGCGCGCATCACAAAGACATCGACGCATTT45230.235308807042096No Hit
CTACAACACCGTCCTCCCACGTGCCCGGGTCCGTGTTGCTTGGTCTTCGTC36780.19134773210277006No Hit
CATCACAAAGACATCGACGCATTTACCGAACAACTCCGCGACATTTTTGTA36180.18822623565737415No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT29320.15253712629834743No Hit
ATACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTC28170.14655425811133857RNA PCR Primer, Index 33 (95% over 23bp)
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT26710.13895861676087518No Hit
ACGCAGAGTACATGGGTTTATCAATAACCTACAAAAATGTCGCGGAGTTGT24620.12808540414274602No Hit
ATCTACAACACCGTCCTCCCACGTGCCCGGGTCCGTGTTGCTTGGTCTTCG23530.12241468560027678No Hit
ACACATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTG23160.12048976279228263RNA PCR Primer, Index 33 (96% over 25bp)
GTTTATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAAATGCGTCG22500.11705611670234711No Hit
CATCTCCGAGCCCACGAGACAAGAGGCAATCTCGTATGCCGTCTTCTGCTT21240.11050097416701567RNA PCR Primer, Index 33 (96% over 28bp)
TCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAAATGCGTCGATGTC20180.10498633044681621No Hit
GACGAAGACCAAGCAACACGGACCCGGGCACGTGGGAGGACGGTGTTGTAG19990.10399785657244083No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)