FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004968457

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004968457
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences392118
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT93852.3934121871477463No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64821.6530738196155237No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT64481.6444029603333692No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT28460.7258019269709628No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10880.27746749702895557No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10410.2654813091977415No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT10160.25910567737262763No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9330.23793857971324958No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT7620.1943292580294707No Hit
ATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAAATGCGTCGATGT6890.17571241310013822No Hit
ATCAATAACCTACAAAAATGTCGCGCGCATCACAAAGACATCGACGCATTT6420.16372622526892416No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT6350.16194104835789228No Hit
TCACAAAGACATCGACGCATTTACCGAACAACTCCGCGACATTTTTGTAGG6340.1616860230848877No Hit
GTACATGGGTTTATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAA5820.14842470888865086No Hit
CATCACAAAGACATCGACGCATTTACCGAACAACTCCGCGACATTTTTGTA5100.13006288923232293No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4760.12139202995016807Clontech SMART CDS Primer II A (100% over 21bp)
TCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4150.10583548829689023No Hit
ACGCAGAGTACATGGGTTTATCAATAACCTACAAAAATGTCGCGGAGTTGT4090.1043053366588629No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)