FastQCFastQC Report
Fri 2 Sep 2022
EGAF00004968469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004968469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences842466
Sequences flagged as poor quality0
Sequence length51
%GC41

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189982.2550464944579365No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT140561.6684352840352012No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT138931.6490873222183449No Hit
ACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT61410.7289314939712701No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24290.2883202408168401No Hit
GCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23460.2784682111800358No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT23070.27383894424226024No Hit
AACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT19200.22790237232125687No Hit
ATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT16240.19276742325506313No Hit
ATCAATAACCTACAAAAATGTCGCGCGCATCACAAAGACATCGACGCATTT13370.15870076655912524No Hit
ATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAAATGCGTCGATGT13340.15834466910237327No Hit
TCACAAAGACATCGACGCATTTACCGAACAACTCCGCGACATTTTTGTAGG13010.15442759707810166No Hit
GTACATGGGTTTATCAATAACCTACAAAAATGTCGCGGAGTTGTTCGGTAA11780.13982760135127115No Hit
CATCACAAAGACATCGACGCATTTACCGAACAACTCCGCGACATTTTTGTA9440.11205199972461796No Hit
GTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9130.10837232600484767Clontech SMART CDS Primer II A (100% over 21bp)

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)