FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969175

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969175
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15221731
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT279800.18381615073870375No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN265690.17454650854098No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT169170.11113716304669949No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT156100.10255075457581007No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA152400.048.4837151
GTATCAA368000.039.5184251
TCAACGC534150.026.8653414
CAACGCA538400.026.6660145
TATCAAC545350.026.427592
ATCAACG545600.026.3331053
AACGCAG578750.024.8539646
CGCAGAG593550.024.1989298
ACGCAGA606200.023.7500617
AGAGTAC616350.023.30383711
CAGAGTA640500.022.5157210
GCAGAGT655150.022.0330379
AGTACGG386700.021.93212513
GAGTACG388850.021.83699412
GTACGGG405900.020.89468614
TACGGGA171150.019.76212315
TACGGGG227900.019.53406115
GAGTACT304200.019.29206812
AGTACTT320700.018.25712813
GTACTTT339750.017.30361614
Produced by FastQC (version 0.11.5)