FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969176

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969176
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15221731
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN266790.17526915959820863No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT189450.12446022071996936No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA151250.049.3402861
GTATCAA375300.040.0512051
TCAACGC541050.027.3248714
CAACGCA543450.027.1754595
TATCAAC549550.027.1465262
ATCAACG552800.026.7787673
AACGCAG580100.025.5573546
CGCAGAG594600.024.9166778
ACGCAGA605500.024.496427
AGAGTAC617250.024.05193911
CAGAGTA640350.023.28542110
GCAGAGT650850.022.8731339
AGTACGG414900.022.45963713
GAGTACG420400.022.24652512
GTACGGG434500.021.32121814
GAGTACT268450.020.37541812
TACGGGA179900.020.31441515
TACGGGG242600.019.66903915
AGTACTT285350.019.18085913
GTACTTT299550.018.1693914
Produced by FastQC (version 0.11.5)