FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969178

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969178
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences20542814
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN553030.26920849305260713No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT293360.14280419420630494No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA224450.048.9686621
GTATCAA562050.040.4350131
TCAACGC773700.028.9520384
CAACGCA778000.028.7832535
TATCAAC785000.028.7635612
ATCAACG798800.028.1333663
AACGCAG838600.026.7911016
CGCAGAG856900.026.2026718
ACGCAGA885250.025.4092547
AGAGTAC883650.025.35231211
CAGAGTA907400.024.74079910
GCAGAGT924000.024.3291599
AGTACGG574750.024.26939613
GAGTACG576800.024.23004312
GTACGGG598350.023.2614814
TACGGGG354000.022.46879615
GAGTACT388700.021.6445312
TACGGGA224950.021.09514215
AGTACTT407050.020.66873213
GTACTTT420350.019.98272514
Produced by FastQC (version 0.11.5)