FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969179

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969179
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13989719
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT473790.3386701334029654No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN355960.2544439956227855No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT311760.22284936530891009No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT292500.20908211237123492No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA258200.054.96641
GTATCAA666200.040.454331
TATCAAC1019300.026.4058972
ATCAACG1027550.026.1630463
TCAACGC1034850.025.968314
CAACGCA1049400.025.6306045
AACGCAG1090400.024.709886
CGCAGAG1125200.023.9655558
ACGCAGA1126300.023.9391757
AGAGTAC1163850.023.09222611
CAGAGTA1185950.022.74373810
GCAGAGT1187500.022.7281559
GAGTACG762400.021.41112712
AGTACGG763250.021.3785813
TACGGGA318050.020.31758915
GTACGGG801500.020.29974414
GAGTACT520000.020.2601812
ACGGGAT100850.020.0628816
AGTACTT536050.019.59739313
TACGGGG388550.018.95892115
Produced by FastQC (version 0.11.5)