FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969182

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969182
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14317673
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT202280.14127994123067344No Hit
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN185890.12983255030339078No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA177600.050.6616861
GTATCAA437100.039.979911
TCAACGC608000.028.2777674
TATCAAC616000.028.0710242
CAACGCA613450.028.0278915
ATCAACG615250.027.9934733
AACGCAG647250.026.674446
CGCAGAG672400.025.6915078
ACGCAGA680950.025.3647
AGAGTAC691500.024.91805611
CAGAGTA713750.024.21284310
GCAGAGT725350.023.9149519
GAGTACG473700.023.28922312
AGTACGG472150.023.2715313
GTACGGG490400.022.30124514
GAGTACT289700.021.49133312
TACGGGA173850.021.0085515
TACGGGG284800.020.65058115
AGTACTT302600.020.4283613
GTACTTT311550.019.80877914
Produced by FastQC (version 0.11.5)