FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969183

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969183
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12392919
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN461210.3721560675091962No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT254340.2052300995431343No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT164780.13296302509521768No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT125800.10150957978503693No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA203500.051.7109381
GTATCAA470950.037.6542171
ATCAACG662700.026.6900793
TCAACGC662950.026.6693024
TATCAAC665200.026.6211572
CAACGCA669100.026.4343595
AACGCAG695900.025.4642756
ACGCAGA722650.024.5160687
CGCAGAG723700.024.4713768
AGAGTAC750800.023.51699811
GCAGAGT770300.023.0040449
CAGAGTA771450.022.9265110
GAGTACG534650.021.96842812
AGTACGG535250.021.95644413
GTACGGG554900.021.13684514
ACGGGTA60800.020.5200116
TACGGGT154700.020.18335315
GAGTACT296150.019.92592812
TACGGGA117800.019.82259615
GTGGTAT48750.019.7791861
Produced by FastQC (version 0.11.5)