FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969185

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969185
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences22402645
Sequences flagged as poor quality0
Sequence length35-76
%GC44

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN1261780.5632281366776111No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT827380.369322461700393No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT500490.2234066557765835No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT460550.205578403800087No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA391150.052.5785681
GTATCAA978550.039.557681
TATCAAC1403400.027.4876542
ATCAACG1416500.027.2113153
TCAACGC1421650.027.084444
CAACGCA1440050.026.7475055
AACGCAG1495550.025.7571166
ACGCAGA1558850.024.7272977
CGCAGAG1559200.024.7132558
AGAGTAC1629150.023.6361711
GCAGAGT1649100.023.4104399
CAGAGTA1658150.023.2507310
GCCGTCG86500.022.56504870
AGTACGG983050.022.41179713
GAGTACG988950.022.29477312
TCGTATG20500.022.16622540
GTACGGG1032600.021.34271414
TACGGGA439200.021.15240115
GAGTACT777700.021.14606712
AGTACTT804300.020.42619313
Produced by FastQC (version 0.11.5)