FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969189

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969189
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15541825
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN245760.15812814775613546No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA136600.045.3044431
GTATCAA332600.039.774881
TCAACGC450350.029.0686874
CAACGCA450700.029.015785
TATCAAC469850.028.096682
ATCAACG470550.027.9010093
CGCAGAG498350.026.2610868
AACGCAG500600.026.1779276
ACGCAGA519500.025.2835987
AGAGTAC517400.025.20866211
AGTACGG404950.024.33338413
CAGAGTA539000.024.31222710
GAGTACG405950.024.23971412
GCAGAGT552750.023.7505959
GTACGGG418200.023.46465114
TACGGGG261050.021.97988715
TACGGGA147150.021.765815
GCGTATA33800.019.3815953
GAGTACT166850.019.33866912
ACGGGAG81650.018.27778416
Produced by FastQC (version 0.11.5)