FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969190

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969190
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences15541825
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN246970.15890669210340483No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA147000.044.723211
GTATCAA348000.040.117891
CAACGCA464750.029.5235845
TCAACGC464500.029.5212734
TATCAAC486050.028.4215132
ATCAACG484150.028.4036523
AACGCAG512550.026.8555366
CGCAGAG514000.026.7857998
ACGCAGA533600.025.8593777
AGAGTAC534750.025.7784511
AGTACGG421250.025.17822313
GAGTACG423500.025.06046312
CAGAGTA555850.024.9040210
GCAGAGT565400.024.5194519
GTACGGG435850.024.2959614
TACGGGG264200.023.25160815
TACGGGA158150.022.16849715
GAGTACT167550.018.9520412
ACGGGGA124850.018.61169216
ACGGGAG87650.018.07553316
Produced by FastQC (version 0.11.5)