FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969192

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969192
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences16449923
Sequences flagged as poor quality0
Sequence length35-76
%GC43

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN763460.4641115949296541No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT327020.1987972831240608No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT206240.12537444704148465No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT170170.10344729273200853No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA284700.052.333261
GTATCAA701450.038.9159741
TATCAAC1032700.026.277882
ATCAACG1031400.026.2170663
TCAACGC1037550.026.0193964
CAACGCA1048900.025.7371585
AACGCAG1084900.024.9040896
ACGCAGA1118650.024.1553257
CGCAGAG1123950.024.0386168
AGAGTAC1160200.023.28760711
CAGAGTA1188550.022.79637710
GCAGAGT1187850.022.7873849
GAGTACG815300.021.44939412
AGTACGG815950.021.4199713
GTACGGG854500.020.35663214
TACGGGA330350.020.14763615
GAGTACT476450.019.91973312
ACGGGAT115900.019.10415316
AGTACTT496900.018.97288513
TACGGGG391200.018.95694515
Produced by FastQC (version 0.11.5)