FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969194

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969194
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences13650940
Sequences flagged as poor quality0
Sequence length35-76
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN720540.5278317830127449No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT276260.20237434198670567No Hit
GGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGGG204470.14978455696091258No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT188910.1383860745120849No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA232750.050.691231
GTATCAA540500.037.5087281
ATCAACG721600.027.7869873
TATCAAC725250.027.765312
TCAACGC722200.027.69694
CAACGCA728800.027.4567575
AACGCAG759900.026.3890556
ACGCAGA795450.025.2135937
CGCAGAG796450.025.2027518
AGAGTAC811650.024.628111
CAGAGTA834750.024.05827910
GCAGAGT843400.023.8629579
AGTACGG536250.022.71272713
GAGTACG540000.022.60409412
GTGGTAT60950.022.2808111
GAGTACT348700.022.22548712
GTACGGG553700.021.91881614
AGTACTT358800.021.47942213
ACGGGTA68450.020.92239816
GTACTTT369500.020.84848414
Produced by FastQC (version 0.11.5)