FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969195

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969195
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14159788
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN268030.18928955716003656No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT225050.15893599536942218No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA145500.048.386771
GTATCAA350500.039.2901571
TCAACGC487850.027.8463484
CAACGCA490150.027.7021065
TATCAAC499450.027.337832
ATCAACG500700.027.1730983
AACGCAG521700.026.0854456
CGCAGAG540000.025.2004598
ACGCAGA552200.024.6683987
AGAGTAC570050.023.80089811
CAGAGTA589600.023.08063710
GCAGAGT594850.022.956929
AGTACGG374250.022.71240213
GAGTACG375850.022.62467812
GTACGGG389100.021.80183214
TACGGGG250400.021.03864515
GAGTACT251250.020.11474612
TACGGGA128000.020.02081715
AGTACTT265800.019.1032313
GTACTTT279500.018.10614
Produced by FastQC (version 0.11.5)