FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969196

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969196
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences14159788
Sequences flagged as poor quality0
Sequence length35-76
%GC45

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[WARN]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN275150.1943178810304222No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT153090.10811602546591799No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGTATCA143250.045.7154581
GTATCAA342550.039.149381
TCAACGC478250.027.65784
CAACGCA478950.027.5922875
ATCAACG489950.027.0649743
TATCAAC492100.026.9980852
AACGCAG510200.025.9748466
CGCAGAG528550.025.0598538
ACGCAGA540550.024.5286737
AGAGTAC555200.023.84472311
CAGAGTA570900.023.2722910
GCAGAGT580900.022.8716669
AGTACGG372850.022.86024913
GAGTACG377150.022.74356812
GTACGGG387550.021.99330114
TACGGGG248200.021.201615
GAGTACT229400.020.22873112
TACGGGA129600.019.65962815
AGTACTT246150.018.76953513
GTACTTT251950.018.2566814
Produced by FastQC (version 0.11.5)