FastQCFastQC Report
Sat 4 Jun 2022
EGAF00004969206

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969206
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences69078915
Sequences flagged as poor quality0
Sequence length101
%GC46

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[OK]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCACACGTCTGAACTCCAGTCACATTCAGAAATCTCGTAT10643371.5407552362395964TruSeq Adapter, Index 13 (97% over 38bp)
AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTT8972701.2989057514872664Clontech SMART CDS Primer II A (100% over 26bp)
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7046041.0199986493707958No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3867850.559917595694721No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)