FastQCFastQC Report
Thu 12 May 2022
EGAF00004969277

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969277
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences34687185
Sequences flagged as poor quality0
Sequence length100
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GATCGGAAGAGCGTCGTGTAGGGAAAGAGTGTAGATCTCGGTGGTCGCCG2545620.7338790968480147Illumina Single End PCR Primer 1 (100% over 50bp)
CTAACACGTGCGCGAGTCGGGGGCTCGCACGAAAGCCGCCGTGGCGCAAT524120.15109902980019854No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)