FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969302

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969302
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences44938
Sequences flagged as poor quality0
Sequence length239
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC824418.34527571320486No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC692515.410120610619074No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC523411.647158307000756No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC43889.764564511104187No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT33107.365703858649695No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT28936.437758689750322No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT21634.813298322132717No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT17893.981040544750545No Hit
GTGTCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT3910.8700876763540878No Hit
GTGTCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT3870.8611865236548133No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC3470.7721749966620678No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC3310.7365703858649695No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC2960.6586852997463172No Hit
GTGCCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT2930.6520094352218613No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC2700.6008278072010326No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC2580.5741243491032089No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT2450.5451956028305666No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC2360.5251680092571989No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT2360.5251680092571989No Hit
GTGCCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT2280.5073657038586498No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC2190.48733811028528196No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC2050.456184075837821No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC2000.4450576349637278No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC1780.39610129511771774No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC1650.36717254884507544No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT1620.36049668432061954No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT1600.35604610797098224No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC1510.3360185143976145No Hit
GTGTCAGCAGCCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCG840.1869242066847657No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGACAAGCGTTGTCCGGATTTAT750.16689661311139792No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG670.14909430771284882No Hit
GTGTCAGCCGCCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCG660.14686901953803017No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTGTCCGGATTTAT640.1424184431883929No Hit
GTGCCAGCCGCCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCG560.12461613778984378No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGACAAGCGTTGTCCGGATTTAT510.11348969691575059No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG480.10681383239129467No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG460.10236325604165739No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)