FastQCFastQC Report
Tue 7 May 2024
EGAF00004969312

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969312
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19213
Total Bases4.5 Mbp
Sequences flagged as poor quality0
Sequence length239
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC17579.144849841253318No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC13617.083745380731796No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT10485.454640087440795No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC10005.204809243741217No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT8324.330401290792692No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC8054.18987144121168No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG7944.132618539530526No Hit
GTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT6683.4768125748191334No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC6583.424764482381721No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG6283.2686202050694844No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT5833.0344037891011295No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC5532.878259511788893No Hit
GTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT5312.763753708426586No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT4652.420236298339666No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG4112.13917659917764No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG3671.9101649924530266No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC3651.8997553739655442No Hit
GTGCCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT3621.8841409462343206No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC3011.5666475823661061No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC2991.556237963878624No Hit
GTGCCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT2901.5093946806849527No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC2091.0878051319419144No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC1450.7546973403424764No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC1410.7338781033675116No Hit
GTGTCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT1320.6870348201738407No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC1080.5621193983240514No Hit
GTGTCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT1030.5360953521053454No Hit
GTGCCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT810.4215895487430386No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC810.4215895487430386No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC720.37474626554936763No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC710.3695414563056264No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC710.3695414563056264No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC700.3643366470618852No Hit
GTGCCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT630.32790298235569665No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT600.312288554624473No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC550.28626450840576695No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT480.2498308436995784No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC470.2446260344558372No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG460.23942122521209597No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC460.23942122521209597No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC450.23421641596835474No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT440.22901160672461354No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC430.22380679748087232No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC380.19778275126216624No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT380.19778275126216624No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC350.1821683235309426No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG340.1769635142872014No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT320.16655389579971894No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT320.16655389579971894No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT310.16134908655597774No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC300.1561442773122365No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC290.15093946806849529No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC280.14573465882475406No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT280.14573465882475406No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG270.14052984958101286No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCCATAGCATATGTTAAAGTTGC270.14052984958101286No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC270.14052984958101286No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT260.13532504033727163No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC260.13532504033727163No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGAGCGAGCGTTATCCGGATTCAT260.13532504033727163No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG260.13532504033727163No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC260.13532504033727163No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT240.1249154218497892No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT220.11450580336230677No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGAATTAC220.11450580336230677No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC210.10930099411856554No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTATTCGGAATTAC210.10930099411856554No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCAAGCGTTATCCGGATTTAT200.10409618487482433No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC200.10409618487482433No Hit
GTGTCAGCAGCCGCGGTAACACGATTCACCCAAGTCAATAGAAGCCGGCG200.10409618487482433No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT200.10409618487482433No Hit
GTGTCAGCCGCCGCGGTAACACGATTCACCCAAGTCAATAGAAGCCGGCG200.10409618487482433No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGCAGCCGGCG200.10409618487482433No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)