FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969313

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969313
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences19213
Sequences flagged as poor quality0
Sequence length239
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA3371.7540207151407903No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2981.5510331546348826No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2821.4677562067350232No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2651.3792744495914226No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2541.322021547910269No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2541.322021547910269No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2441.269973455472857No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2361.2283349815229272No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2281.1866965075729974No Hit
GGACTACACGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2191.1398532243793265No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2131.1086243689168793No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1971.0253474210170197No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1931.0045281840420548No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1920.9993233747983136No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1920.9993233747983136No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1880.9785041378233488No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1850.9628897100921251No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1840.9576849008483839No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1760.9160464268984542No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1760.9160464268984542No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1700.8848175714360069No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1680.8744079529485244No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1660.863998334461042No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1630.8483839067298184No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1600.8327694789985947No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1560.8119502420236298No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1550.8067454327798886No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1520.791131005048665No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1510.7859261958049238No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1480.7703117680737002No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1470.7651069588299588No Hit
GGACTACGCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1430.744287721854994No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1400.7286732941237704No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1350.7026492479050642No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1350.7026492479050642No Hit
GGACTACTAGGGTTTCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT1330.6922396294175818No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1320.6870348201738407No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1300.6766252016863582No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1290.671420392442617No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1280.6662155831988757No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1240.645396346223911No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1200.624577109248946No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1190.6193723000052048No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1190.6193723000052048No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1170.6089626815177224No Hit
GGACTACTGGGGTTTCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT1170.6089626815177224No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1160.6037578722739811No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1140.5933482537864987No Hit
GGACTACGGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1080.5621193983240514No Hit
GGACTACGAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1070.5569145890803102No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1060.551709779836569No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1050.5465049705928278No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1050.5465049705928278No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1030.5360953521053454No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1030.5360953521053454No Hit
GGACTACTCGGGTTTCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT1030.5360953521053454No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1020.5308905428616041No Hit
GGACTACAAGGGTTTCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT1020.5308905428616041No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1010.525685733617863No Hit
GGACTACCAGGGTTTCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT1000.5204809243741216No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1000.5204809243741216No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA990.5152761151303805No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA980.5100713058866393No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA970.5048664966428981No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA970.5048664966428981No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA960.4996616873991568No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA960.4996616873991568No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC950.4944568781554156No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA950.4944568781554156No Hit
GGACTACTAGGGTATCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT950.4944568781554156No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA940.4892520689116744No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC940.4892520689116744No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA930.4840472596679332No Hit
GGACTACGGGGGTTTCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT920.47884245042419193No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA920.47884245042419193No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC920.47884245042419193No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA900.4684328319367095No Hit
GGACTACAGGGGTTTCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT890.4632280226929683No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA870.4528184042054859No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA870.4528184042054859No Hit
GGACTACGAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC860.44761359496174463No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA850.44240878571800346No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC850.44240878571800346No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA840.4372039764742622No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA830.431999167230521No Hit
GGACTACAAGGGTATCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT830.431999167230521No Hit
GGACTACGCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC830.431999167230521No Hit
GGACTACGAGGGTTTCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT800.41638473949929733No Hit
GGACTACTCGGGTATCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT800.41638473949929733No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA760.3955655025243325No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC740.3851558840368501No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA740.3851558840368501No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC740.3851558840368501No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA740.3851558840368501No Hit
GGACTACGCGGGTTTCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT720.37474626554936763No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC710.3695414563056264No Hit
GGACTACCGGGGTTTCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT710.3695414563056264No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC700.3643366470618852No Hit
GGACTACTGGGGTATCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT690.35913183781814395No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC690.35913183781814395No Hit
GGACTACACGGGTTTCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT680.3539270285744028No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC680.3539270285744028No Hit
GGACTACGGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC680.3539270285744028No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA680.3539270285744028No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA670.34872221933066155No Hit
GGACTACGGGGGTATCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT650.3383126008431791No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA650.3383126008431791No Hit
GGACTACCAGGGTATCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT640.3331077915994379No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA630.32790298235569665No Hit
GGACTACGAGGGTATCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT620.3226981731119555No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA600.312288554624473No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA580.30187893613699057No Hit
GGACTACGCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC570.29667412689324935No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC560.2914693176495081No Hit
GGACTACCGGGGTATCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT530.2758548899182845No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA510.26544527143080204No Hit
GGACTACCCGGGTATCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT510.26544527143080204No Hit
GGACTACCCGGGTTTCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT510.26544527143080204No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA510.26544527143080204No Hit
GGACTACACGGGTATCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT490.25503565294331965No Hit
GGACTACAGGGGTATCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT480.2498308436995784No Hit
GGACTACGCGGGTATCTAATCAGGTAGTTAGACCAGCACGTAAAGAGTCT470.2446260344558372No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA440.22901160672461354No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC430.22380679748087232No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA420.2186019882371311No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA410.2133971789933899No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA410.2133971789933899No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA400.20819236974964866No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA390.20298756050590744No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA350.1821683235309426No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA350.1821683235309426No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA340.1769635142872014No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA340.1769635142872014No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA330.17175870504346016No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA320.16655389579971894No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA300.1561442773122365No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA300.1561442773122365No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA280.14573465882475406No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA280.14573465882475406No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA280.14573465882475406No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA280.14573465882475406No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA270.14052984958101286No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA270.14052984958101286No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA260.13532504033727163No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA260.13532504033727163No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA260.13532504033727163No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA250.1301202310935304No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA250.1301202310935304No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA250.1301202310935304No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA250.1301202310935304No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA240.1249154218497892No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA240.1249154218497892No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA240.1249154218497892No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA230.11971061260604798No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA230.11971061260604798No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA220.11450580336230677No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA220.11450580336230677No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA210.10930099411856554No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA210.10930099411856554No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA210.10930099411856554No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTCGCTATTGTGTGTTC210.10930099411856554No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA210.10930099411856554No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA200.10409618487482433No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTTCCTCA200.10409618487482433No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA200.10409618487482433No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)