FastQCFastQC Report
Sat 23 Apr 2022
EGAF00004969315

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969315
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences81758
Sequences flagged as poor quality0
Sequence length239
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA52156.378580689351502No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA44945.496709802098877No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA35134.296827221800925No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA33044.041194745468333No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA32173.9347831404877813No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA32153.9323368966951247No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA30883.777000415861445No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA30053.675481298466205No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA29203.5715159372783094No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA29113.5605078402113555No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA28633.5017979891876023No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA28573.494459257809633No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA28533.4895667702243207No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA27253.3330071674943125No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA26513.242496147166027No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA25393.1055064947772695No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA25093.0688128378874238No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA25023.0602509846131265No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA24723.023557327723281No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA23222.8400890432740526No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA22642.769147973287018No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA22502.752024266738423No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA20582.517184862643411No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA20192.4694831086866116No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA5180.6335771422980014No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA4140.5063724650798698No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA3150.38528339734337924No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2940.3595978375204873No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2910.3559284718315027No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2740.3351353995939235No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2700.3302429120086108No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2690.3290197901122826No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2660.32535042442329803No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2640.32290418063064164No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2580.3155654492526725No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2560.31311920546001615No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2560.31311920546001615No Hit
GGACTACGCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2530.3094498397710316No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2490.30455735218571883No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2490.30455735218571883No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2450.2996648646004061No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2420.2959954989114215No Hit
GGACTACACGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2370.28987988942978055No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2230.2727561828811859No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2140.26174808581423226No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1900.23239316030235574No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1720.21037696616844834No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1720.21037696616844834No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACGGGG12250.0233.000025
AAGGGTA2600.0233.000028
CTAGGGT6700.0233.000027
TGGGGTA3300.0233.000028
ACGGGTA2500.0233.000028
TAGGGTA3150.0233.000028
CTACTAG6700.0233.000024
ACTACTA6700.0233.000023
TACTAGG6700.0233.000025
GACTACC18700.0233.000022
GCGGGTT3250.0233.000028
AGGGGTA3200.0233.08
CTACAGG5500.0233.04
ACCGGGG6350.0233.06
TACAGGG5500.0233.05
CGGGGTT3900.0233.08
GGACTCC100.0017127875233.01
AGGGTTT14850.0233.09
AGGGTTC100.0017127875233.09
GGATTAC100.0017127875233.01
Produced by FastQC (version 0.11.5)