FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969321

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969321
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences76088
Sequences flagged as poor quality0
Sequence length239
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA24863.2672694774471664No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA23423.078014930080959No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA16952.227683734623068No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA15452.030543581116602No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA15432.0279150457365156No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA15412.0252865103564295No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA15302.010829565765955No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA15151.9911155504153086No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA13761.8084323414993164No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA13511.7755756492482389No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA13371.7571759015876356No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA13111.7230049416465145No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA12801.6822626432551784No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA12651.6625486279045316No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA12581.6533487540742298No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA12531.6467774156240143No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA12011.5784354957417726No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA11781.548207338870781No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA11771.546893071180738No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA11251.4785511512984966No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA10891.4312375144569445No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA10791.4180948375565134No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA10761.4141520344863843No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA10461.374724003785091No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA10441.3720954684050048No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA10421.3694669330249185No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA10381.364209862264746No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA10321.3563242561244875No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA9761.2827252654820733No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA9201.2091262748396594No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA9081.193355062559142No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTGA8991.181526653348754No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8931.1736410472084953No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8931.1736410472084953No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8721.1460414257175902No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8691.142098622647461No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8691.142098622647461No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8621.1328987488171591No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8261.085585111975607No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8021.0540426874145727No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA7921.0409000105141415No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA7370.9686152875617705No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA7350.9659867521816844No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA7280.9567868783513827No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6990.9186731153401325No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6550.8608453369782357No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6430.8450741246977185No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6000.7885606140258649No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA5000.6571338450215539No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA4340.5703921774787089No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA3270.4297655346440963No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA3070.4034801808432341No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2940.38639470087267375No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2760.3627378824518978No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2740.3601093470718116No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2740.3601093470718116No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2720.35748081169172535No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA2630.3456524024813374No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2560.33645252865103564No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2510.32988119020082013No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2500.32856692251077696No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2500.32856692251077696No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2490.3272526548207339No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2460.32330985175060456No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2340.30753863947008725No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2310.3035958363999579No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2290.30096730101987174No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2180.28651035642939754No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA2150.28256755335926825No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA2130.279939017979182No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2110.27731048259909574No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2100.2759962149090527No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA2090.27468194721900957No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA2080.27336767952896646No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA2040.268110608768794No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1900.24971086110819052No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1900.24971086110819052No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1880.2470823257281043No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1870.24576805803806118No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1860.2444537903480181No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1850.24313952265797495No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1790.23525391651771635No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1750.22999684575754387No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1690.22211123961728527No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1590.20896856271685416No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1590.20896856271685416No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1550.20371149195668173No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1490.19582588581642307No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1490.19582588581642307No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1370.1800546735359058No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1340.17611187046577648No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1310.17216906739564714No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1270.1669119966354747No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1260.16559772894543162No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1210.15902639049521608No Hit
GGACTACACGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1210.15902639049521608No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCA1170.15376931973504362No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCA1090.14325517821469877No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCA880.1156555567237935No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCA800.10514141520344865No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCTAGCTTTCGCTCCTCA770.10119861213331932No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)