FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969324

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969324
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55450
Sequences flagged as poor quality0
Sequence length239
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT1413825.496844003606856No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT1094619.740306582506765No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT826614.90712353471596No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT651111.742110009017132No Hit
GTGTCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT14872.6816952209197478No Hit
GTGTCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT10721.9332732191163209No Hit
GTGTCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT10061.8142470694319206No Hit
GTGCCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT8791.5852119026149685No Hit
GTGTCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT7721.3922452660054103No Hit
GTGCCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT6131.1055004508566275No Hit
GTGCCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT5410.975653742110009No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC5330.9612263300270515No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT5110.9215509467989179No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC4680.8440036068530208No Hit
GTGCCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT4380.7899008115419296No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT3720.6708746618575293No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC3220.5807033363390441No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC2760.49774571686203783No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT2740.49413886384129846No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT2450.44183949504057707No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT2150.387736699729486No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT1690.3047790802524797No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT1430.25788999098286747No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAACCGGCG1220.2200180342651037No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT1030.18575293056807934No Hit
GTGTCAGCAGCCGCGGTAATACCTAGGTCGCAAGCGTTATCCGGAATTAT960.17312894499549142No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAACCGGCG830.1496844003606853No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAACCGGCG600.10820559062218214No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAACCGGCG570.10279531109107304No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)