FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969328

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969328
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences17616
Sequences flagged as poor quality0
Sequence length239
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC12587.141235240690282No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC10565.994550408719346No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC7714.3767029972752045No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC7674.353996366939146No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC7074.013396911898274No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC6803.860127157129882No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC4832.741825613079019No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC4722.679382379654859No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT4712.6737057220708444No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT4552.5828792007266124No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT4512.560172570390554No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC4272.4239327883742052No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT4082.316076294277929No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC3762.134423251589464No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT2871.6292007266121709No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT2811.5951407811080835No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC2751.5610808356039962No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC2731.5497275204359673No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT2671.5156675749318802No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT2501.419164396003633No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT2371.345367847411444No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT1981.1239782016348774No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG1881.067211625794732No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG1760.9990917347865577No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT1670.9480018165304268No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT1490.8458219800181653No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG1320.7493188010899182No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT1210.6868755676657584No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT1080.6130790190735695No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGAATTAC970.5506357856494096No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG890.5052225249772934No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC850.48251589464123523No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGAATTAC820.4654859218891917No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC800.4541326067211626No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT680.38601271571298823No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGAATTAC660.3746594005449591No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC630.3576294277929155No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATCAT620.351952770208901No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT610.34627611262488645No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTGTCCGGAATTAT600.3405994550408719No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC600.3405994550408719No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT590.3349227974568574No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATCAT580.3292461398728429No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT580.3292461398728429No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT570.32356948228882837No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC550.3122161671207993No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT550.3122161671207993No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGAATTAC540.30653950953678477No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT530.30086285195277024No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT530.30086285195277024No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATCAT510.2895095367847411No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTGTCCGGAATTAT490.27815622161671205No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC480.2724795640326975No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGAATCAT460.26112624886466845No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGACCGAGCGTTGTCCGGAATCAT440.24977293369663942No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT420.23841961852861035No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGGGCTAGCGTTGTTCGGAATTAC410.23274296094459584No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC400.2270663033605813No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT380.21571298819255225No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGCGCGAGCGTTGTCCGGAATTAT360.20435967302452315No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT360.20435967302452315No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT360.20435967302452315No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT350.19868301544050862No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC340.19300635785649412No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGGGCTAGCGTTGTTCGGAATTAC330.18732970027247955No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAT330.18732970027247955No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTGTCCGGAATTAT320.18165304268846502No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT320.18165304268846502No Hit
GTGCCAGCAGCCGCGGTAATACGTAAGGTCCGAGCGTTGTCCGGAATCAT320.18165304268846502No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAT310.1759763851044505No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGT290.16462306993642145No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGACCGAGCGTTGTCCGGAATCAT290.16462306993642145No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT290.16462306993642145No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT290.16462306993642145No Hit
GTGTCAGCCGCCGCGGTAATACGTAAGGTCCGAGCGTTGTCCGGAATCAT290.16462306993642145No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC280.15894641235240692No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGGGCTAGCGTTGTTCGGAATTAC280.15894641235240692No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC270.15326975476839239No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT260.14759309718437782No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT260.14759309718437782No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT260.14759309718437782No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT250.1419164396003633No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT250.1419164396003633No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAT250.1419164396003633No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCCATAGCGTATATTAAAGTTGT240.13623978201634876No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC240.13623978201634876No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC230.13056312443233423No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGTCCCGAGCGTTGTTCGGAATCAC230.13056312443233423No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCCATAGCGTATATTAAAGTTGT230.13056312443233423No Hit
GTGTCAGCAGCCGCGGTAATACGTAAGGTCCGAGCGTTGTCCGGAATCAT230.13056312443233423No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC230.13056312443233423No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGT230.13056312443233423No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC230.13056312443233423No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC220.12488646684831971No Hit
GTGTCAGCCGCCGCGGTAATACGTAAGGGGCGAGCGTTGTCCGGAATTAT210.11920980926430517No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC210.11920980926430517No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGT200.11353315168029066No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC200.11353315168029066No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC200.11353315168029066No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT200.11353315168029066No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCACTAGCGTATATTAAAGTTGT190.10785649409627612No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGACCGAGCGTTGTCCGGAATCAT190.10785649409627612No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT190.10785649409627612No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC180.10217983651226158No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAT180.10217983651226158No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGT180.10217983651226158No Hit
GTGCCAGCCGCCGCGGTAATACGTAAGGTCCGAGCGTTGTCCGGAATCAT180.10217983651226158No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC180.10217983651226158No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)