FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969334

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969334
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences95775
Sequences flagged as poor quality0
Sequence length239
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAACCGGCG1939520.250587314017228No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAACCGGCG1456415.206473505612111No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAACCGGCG1086311.342208300704778No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAACCGGCG85808.958496476115897No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACACCGGCG16901.7645523362046462No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACACCGGCG15791.6486557034716784No Hit
GTGTCAGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCGGATTTAT15551.6235969720699555No Hit
GTGTCAGCCGCCGCGGTAATACGTATGTCCCGAGCGTTATCCGGATTTAT12111.2644218219785957No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACACCGGCG9741.016966849386583No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACACCGGCG9611.0033933698773165No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC9230.9637170451579222No Hit
GTGCCAGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCGGATTTAT9110.9511876794570608No Hit
GTGCCAGCCGCCGCGGTAATACGTATGTCCCGAGCGTTATCCGGATTTAT7700.8039676324719395No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC7570.7903941529626729No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC7170.7486296006264683No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC6270.6546593578700078No Hit
GTGTCAGCAGCCGCGGTCACACGATTAACCCAAGTCAATAGAAACCGGCG5730.5982772122161316No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGCAACCGGCG5240.5471156356042809No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC5180.5408509527538501No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC4470.4667188723570869No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC4400.4594100756982511No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGCAACCGGCG3960.413469068128426No Hit
GTGCCAGCAGCCGCGGTCACACGATTAACCCAAGTCAATAGAAACCGGCG3680.38423388149308274No Hit
GTGTCAGCCGCCGCGGTCACACGATTAACCCAAGTCAATAGAAACCGGCG3360.350822239624119No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGCAACCGGCG3240.33829287392325763No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC3240.33829287392325763No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAT3140.32785173583920646No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT2720.28399895588619156No Hit
GTGTCAGCAGCCGCGGTAACACGATTCACCCAAGTCAATAGAAACCGGCG2690.28086661446097627No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCACTAGAAACCGGCG2660.27773427303576087No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGCAACCGGCG2640.27564604541895066No Hit
GTGCCAGCCGCCGCGGTCACACGATTAACCCAAGTCAATAGAAACCGGCG2520.2631166797180893No Hit
GTGTCAGCAGCCGCGGTAACACGATTACCCCAAGTCAATAGAAACCGGCG2370.24745497259201252No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCACTAGAAACCGGCG2330.24327851735839204No Hit
GTGTCAGCCGCCGCGGTAACACGATTCACCCAAGTCAATAGAAACCGGCG2320.24223440354998693No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAT2280.23805794831636648No Hit
GTGTCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT2050.21404333072304882No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT2000.20882276168102323No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCACTAGAAACCGGCG1950.20360219263899768No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCACTAGAAACCGGCG1860.1942051683633516No Hit
GTGTCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT1840.19211694074654137No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAT1820.19002871312973113No Hit
GTGTCAGCCGCCGCGGTAACACGATTACCCCAAGTCAATAGAAACCGGCG1770.18480814408770557No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT1540.16079352649438788No Hit
GTGCCAGCAGCCGCGGTAACACGATTCACCCAAGTCAATAGAAACCGGCG1530.15974941268598275No Hit
GTGCCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT1450.15139650221874185No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCCATAGAAACCGGCG1440.1503523884103367No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAT1390.14513181936831115No Hit
GTGCCAGCCGCCGCGGTAACACGATTCACCCAAGTCAATAGAAACCGGCG1360.14199947794309578No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT1320.13782302270947533No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAACCGGCA1310.13677890890107022No Hit
GTGCCAGCAGCCGCGGTAACACGATTACCCCAAGTCAATAGAAACCGGCG1190.12424954320020884No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAACCCGCG1110.1158966327329679No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGATTTAT1100.11485251892456277No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC1090.11380840511615767No Hit
GTGCCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT1090.11380840511615767No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTGTCCGGAATTAT1090.11380840511615767No Hit
GTGTCAGCAGCCGCGGTAACACGCTTAACCCAAGTCAATAGAAACCGGCG980.10232315322370139No Hit
GTGCCAGCCGCCGCGGTAACACGATTACCCCAAGTCAATAGAAACCGGCG970.10127903941529626No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGATTTAT960.10023492560689115No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)