FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969340

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969340
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61400
Sequences flagged as poor quality0
Sequence length239
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT900714.669381107491859No Hit
GTGTCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT724811.80456026058632No Hit
GTGCCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT50278.18729641693811No Hit
GTGCCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT42956.995114006514658No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAT33735.493485342019544No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAT27844.534201954397394No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT25394.135179153094462No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT22203.6156351791530943No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT20593.3534201954397393No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT18813.063517915309446No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAT18132.9527687296416936No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAT15412.509771986970684No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT14842.4169381107491854No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT13492.197068403908795No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT11931.9429967426710097No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT11111.8094462540716612No Hit
GTGTCAGCAGCCGCGGTAATACCTAGGTCGCAAGCGTTATCCGGAATTAT9561.5570032573289903No Hit
GTGTCAGCCGCCGCGGTAATACCTAGGTCGCAAGCGTTATCCGGAATTAT7031.1449511400651466No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT7031.1449511400651466No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT6060.9869706840390879No Hit
GTGCCAGCAGCCGCGGTAATACCTAGGTCGCAAGCGTTATCCGGAATTAT5330.8680781758957654No Hit
GTGCCAGCCGCCGCGGTAATACCTAGGTCGCAAGCGTTATCCGGAATTAT4030.6563517915309446No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT3800.6188925081433224No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTATCCGGAATTAT3290.5358306188925082No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC3270.5325732899022801No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC2840.46254071661237783No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC1910.3110749185667752No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATTCGGAATCAT1800.2931596091205212No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC1780.2899022801302932No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATTCGGAATCAT1660.2703583061889251No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT1280.20846905537459282No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT1280.20846905537459282No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAT1160.18892508143322476No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCGCAAGCGTTATCCGGAATTAT1130.18403908794788273No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATTCGGAATCAT1100.1791530944625407No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT1050.17100977198697068No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT1040.16938110749185667No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT980.15960912052117263No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATTCGGAATCAT890.1449511400651466No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT850.13843648208469056No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAT780.1270358306188925No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT780.1270358306188925No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCGCAAGCGTTATCCGGAATTAT730.11889250814332247No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT710.11563517915309446No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAT680.11074918566775244No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTACTCGGAATCAC680.11074918566775244No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT660.10749185667752444No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG640.10423452768729641No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTACTCGGAATCAC620.10097719869706841No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)