FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969344

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969344
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences33227
Sequences flagged as poor quality0
Sequence length239
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT896726.987088813314475No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT713321.467481265236103No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT509215.32488638757637No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT403912.155776928401602No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC7952.3926324976675595No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC6161.8539139856141091No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC4701.4145122942185573No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT4691.41150269359256No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT3951.1887922472687873No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC3811.1466578385048303No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT2660.8005537665151835No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT2170.6530833358413338No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT1790.5387185120534506No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT1500.45144009389953954No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG1120.33707527011165617No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT990.2979504619736961No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG860.258825653835736No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT850.2558160532097391No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG750.22572004694976977No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT730.2197008456977759No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG650.19562404068980047No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT600.1805760375598158No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT470.14145122942185573No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT420.12640322629187106No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT410.12339362566587413No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC380.11436482378788335No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT380.11436482378788335No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT370.11135522316188641No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT360.1083456225358895No Hit
GTGTCAGCAGCCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCG350.10533602190989255No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT340.10232642128389562No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)