FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969350

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969350
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7881
Sequences flagged as poor quality0
Sequence length239
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC79410.074863595990356No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC5817.372160893287654No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC5156.5347037178023095No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC4205.329272934906737No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAACCGGCG2513.1848750158609316No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAACCGGCG2232.8295901535338155No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAACCGGCG1802.2839741149600306No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC1712.169775409212029No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC1682.1317091739626948No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC1491.8906230173835807No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC1381.7510468214693566No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAACCGGCG1311.6622256058875777No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC1251.58609313538891No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC1221.5480269001395761No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC1121.4211394493084635No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTCCGAGCGTTGTCCGGAATTAT1101.3957619591422408No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT1011.2815632533942392No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC961.218119527978683No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC961.218119527978683No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC911.1546758025631265No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTCCGAGCGTTGTCCGGAATTAT881.1166095673137926No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGATCCAAGCGTTGTCCGGATTTAT851.0785433320644588No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC851.0785433320644588No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC841.0658545869813474No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTCCGAGCGTTGTCCGGAATTAT821.040477096815125No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC811.0277883517320137No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT710.9009009009009009No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTCCGAGCGTTGTCCGGAATTAT700.8882121558177896No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT690.8755234107346783No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC680.8628346656515671No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT670.8501459205684557No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC660.8374571754853445No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC660.8374571754853445No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC640.8120796853191219No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC610.7740134500697881No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC440.5583047836568963No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGATCCAAGCGTTGTCCGGATTTAT440.5583047836568963No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGATCCAAGCGTTGTCCGGATTTAT430.545616038573785No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC410.5202385484075625No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT360.4567948229920061No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGATCCAAGCGTTGTCCGGATTTAT320.40603984265956095No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC310.3933510975764497No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC310.3933510975764497No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT310.3933510975764497No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT300.3806623524933384No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT280.35528486232711587No Hit
GTGTCAGCAGCCGCGGTAGTCCGTACAAGAGTGAAGATCGGAAGAGCGTC270.34259611724400457No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC270.34259611724400457No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT260.3299073721608933No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT240.30452988199467074No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC240.30452988199467074No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACACCGGCG230.29184113691155944No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT230.29184113691155944No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACACCGGCG220.27915239182844814No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC220.27915239182844814No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACACCGGCG220.27915239182844814No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC220.27915239182844814No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT210.26646364674533685No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT210.26646364674533685No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT200.2537749016622256No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCGAGCGTTAATCGGATTTAC190.24108615657911434No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC190.24108615657911434No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT180.22839741149600304No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT170.21570866641289177No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGATGCAAGCGTTATCCGGAATGAT160.20301992132978047No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT150.1903311762466692No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT150.1903311762466692No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT150.1903311762466692No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC150.1903311762466692No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC140.17764243116355793No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCGAGCGTTAATCGGATTTAC140.17764243116355793No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC130.16495368608044664No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCGAGCGTTAATCGGATTTAC130.16495368608044664No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC120.15226494099733537No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACACCGGCG120.15226494099733537No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT120.15226494099733537No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC120.15226494099733537No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTAC120.15226494099733537No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT110.13957619591422407No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC110.13957619591422407No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC110.13957619591422407No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGATTTAT110.13957619591422407No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT110.13957619591422407No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC100.1268874508311128No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT100.1268874508311128No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC100.1268874508311128No Hit
GTGCCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT100.1268874508311128No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC90.11419870574800152No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC90.11419870574800152No Hit
GTGCCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT90.11419870574800152No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT90.11419870574800152No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGCAACCGGCG90.11419870574800152No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGCAACCGGCG90.11419870574800152No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT80.10150996066489024No Hit
GTGTCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT80.10150996066489024No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT80.10150996066489024No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGATGCAAGCGTTATCCGGAATGAT80.10150996066489024No Hit
GTGTCAGCAGCCGCGGTAAGACGGGGGGGGCAAGTGTTCTTCGGAATGAC80.10150996066489024No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGATTTAT80.10150996066489024No Hit
GTGTCAGCAGCCGCGGTAATACGGGGGGGGCCGGCGTTATTCGGTATATC80.10150996066489024No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC80.10150996066489024No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)