FastQCFastQC Report
Wed 8 May 2024
EGAF00004969354

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969354
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences50867
Total Bases12.1 Mbp
Sequences flagged as poor quality0
Sequence length239
%GC54

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC681613.39965006782393No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC643912.658501582558438No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC546210.737806436392946No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC516510.15393083924745No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT18453.627105982267482No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT17213.383333005681483No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT15002.9488666522499853No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT14142.779798297520986No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCTGGCGTTATCCGGATTTAT8811.7319676804214912No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCTGGCGTTATCCGGATTTAT8411.6533312363614918No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCTGGCGTTATCCGGATTTAT7161.407592348673993No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCTGGCGTTATCCGGATTTAT7061.387933237658993No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT6871.3505809267304931No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT6431.2640808382644937No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT5771.1343307055654943No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT5691.1186034167534944No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT5531.0871488391294946No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT5481.0773192836219947No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT5211.0242396838814949No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT5101.002614661764995No Hit
GTGTCAGCAGCCGCGGTAATACGTAAGGTCCGAGCGTTGTCCGGAATCAT4780.9397055065169952No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT4700.9239782177049953No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT4380.8610690624569958No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT4160.8178190182239959No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT4120.8099553738179959No Hit
GTGTCAGCCGCCGCGGTAATACGTAAGGTCCGAGCGTTGTCCGGAATCAT4040.7942280850059961No Hit
GTGTCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT3590.7057620854384964No Hit
GTGCCAGCCGCCGCGGTAATACGTAAGGTCCGAGCGTTGTCCGGAATCAT3580.7037961743369965No Hit
GTGCCAGCAGCCGCGGTAATACGTAAGGTCCGAGCGTTGTCCGGAATCAT3560.6998643521339964No Hit
GTGTCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT3430.6743075078144967No Hit
GTGCCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT3040.597636974855997No Hit
GTGCCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT2720.5347278196079973No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT2440.4796823087659976No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT2280.4482277311419977No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT1940.38138675369099806No Hit
GTGTCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT1910.3754890203864981No Hit
GTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTATCCGGATTTAT1880.3695912870819982No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATGCGAGCGTTATCCGGATTTAT1870.36762537598049816No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC1820.3577958204729982No Hit
GTGTCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT1790.35189808716849824No Hit
GTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTATCCGGATTTAT1670.32830715395049836No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT1630.3204435095444984No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC1600.3145457762399984No Hit
GTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTATCCGGATTTAT1510.29685257632649853No Hit
GTGCCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT1480.29095484302199853No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC1470.28898893192049857No Hit
GTGCCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT1460.28702302081899855No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT1380.27129573200699864No Hit
GTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTATCCGGATTTAT1350.2653979987024987No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC1300.2555684431949987No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT1090.21428431006349893No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT1070.21035248786049893No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGAATTAT980.19265928794699902No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGAATTAT870.17103426583049913No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGAATTAT770.15137515481549924No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGAATTAT720.1415455993079993No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTCAT540.10615919948099947No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)