FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969360

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969360
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences61634
Sequences flagged as poor quality0
Sequence length239
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC870914.13018788331116No Hit
GTGTCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT740712.0177174935912No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC690911.209721906739786No Hit
GTGTCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT59739.691079598922672No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC48787.91446279650842No Hit
GTGCCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT43367.035078041340818No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC39746.447739883830353No Hit
GTGCCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT34075.527793101210371No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG10791.7506571048447286No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG8901.4440081773047344No Hit
GTGTCAGCAGCCGCGGTAATACCTAGGTCGCAAGCGTTATCCGGAATTAT6881.1162669954895026No Hit
GTGTCAGCCGCCGCGGTAATACCTAGGTCGCAAGCGTTATCCGGAATTAT6110.9913359509361717No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG5970.9686212155628386No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG5420.8793847551676023No Hit
GTGCCAGCAGCCGCGGTAATACCTAGGTCGCAAGCGTTATCCGGAATTAT4160.6749521368076062No Hit
GTGCCAGCCGCCGCGGTAATACCTAGGTCGCAAGCGTTATCCGGAATTAT3140.5094590648018951No Hit
GTGTCAGCAGCCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCG2610.42346756660284907No Hit
GTGTCAGCAGCCGCGGTAATACGTAAGGTCCGAGCGTTGTCCGGAATCAT2190.3553233604828504No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT1950.3163838141285654No Hit
GTGTCAGCCGCCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCG1700.27582178667618523No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT1690.27419930557809No Hit
GTGCCAGCAGCCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCG1620.26284193789142357No Hit
GTGTCAGCCGCCGCGGTAATACGTAAGGTCCGAGCGTTGTCCGGAATCAT1610.26121945679332836No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT1540.24986208910666188No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT1450.23525975922380504No Hit
GTGTCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT1430.23201479702761463No Hit
GTGTCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT1380.2239023915371386No Hit
GTGCCAGCAGCCGCGGTAATACGTAAGGTCCGAGCGTTGTCCGGAATCAT1240.20118765616380568No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC1060.17198299639809195No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT1040.16873803420190156No Hit
GTGCCAGCCGCCGCGGTAATACGTAAGGTCCGAGCGTTGTCCGGAATCAT1000.1622481098095207No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT980.15900314761333031No Hit
GTGCCAGCCGCCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCG980.15900314761333031No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT970.1573806665152351No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT960.1557581854171399No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT960.1557581854171399No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCGCAAGCGTTATCCGGAATTAT950.1541357043190447No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT890.14440081773047345No Hit
GTGCCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT850.13791089333809262No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG840.1362884122399974No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTATCCGGATTCAT810.13142096894571179No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC770.12493104455333094No Hit
GTGCCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT730.11844112016095013No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT720.11681863906285492No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG710.1151961579647597No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCGCAAGCGTTATCCGGAATTAT660.10708375247428367No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC630.10221630917999806No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG620.10059382808190284No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)