FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969374

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969374
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences18666
Sequences flagged as poor quality0
Sequence length239
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC11175.9841422907960995No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG8944.789456766313083No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC8944.789456766313083No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC8074.323368691738991No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG7874.216222007928855No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC7514.023357977070609No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC7484.007285974499089No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC7453.9912139719275688No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG7143.825136612021858No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG6583.525125897353477No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT6453.4554805528768884No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC6033.230472516875603No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC5693.0483231543983713No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT4852.5983070823958No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT4212.2554376942033643No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT3721.992928318868531No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC3201.7143469409621772No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC2431.3018322082931533No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC2101.1250401800064287No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC1860.9964641594342655No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC1710.9161041465766635No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC1510.8089574627665275No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC1350.7232401157184185No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC1320.7071681131468981No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC1280.6857387763848709No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC1200.6428801028608165No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC1120.6000214293367621No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG930.49823207971713274No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC900.4821600771456124No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC880.47144540876459873No Hit
GTGTCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT870.46608807457409196No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC740.3964427300975035No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC700.37501339333547623No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC680.36429872495446264No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC650.3482267223829422No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT610.32679738562091504No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC600.32144005143040827No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC590.3160827172399014No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC590.3160827172399014No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT550.2946533804778742No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT540.28929604628736744No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG530.2839387120968606No Hit
GTGCCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT520.2785813779063538No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG520.2785813779063538No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT520.2785813779063538No Hit
GTGTCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT490.26250937533483343No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG480.25715204114432655No Hit
GTGTCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT450.2410800385728062No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC430.2303653701917926No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC430.2303653701917926No Hit
GTGCCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT420.22500803600128574No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC410.21965070181077895No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATCCGAGCGTTATCCGGATTTAT400.21429336762027215No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCCATAGCATATGTTAAAGTTGC400.21429336762027215No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT390.20893603342976536No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT380.20357869923925856No Hit
GTGCCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT360.19286403085824494No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT350.18750669666773812No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGC350.18750669666773812No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT330.17679202828672452No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT320.17143469409621773No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAC310.16607735990571093No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT310.16607735990571093No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT300.16072002571520413No Hit
GTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT300.16072002571520413No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT290.1553626915246973No Hit
GTGTCAGCAGCCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCG290.1553626915246973No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT290.1553626915246973No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT280.15000535733419051No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT270.14464802314368372No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT270.14464802314368372No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGCAGCCGGCG270.14464802314368372No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGCAGCCGGCG260.1392906889531769No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC260.1392906889531769No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGC260.1392906889531769No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT250.1339333547626701No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT250.1339333547626701No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCCATAGCATATGTTAAAGTTGC250.1339333547626701No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT240.12857602057216327No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT240.12857602057216327No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT230.12321868638165648No Hit
GTGCCAGCCGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT230.12321868638165648No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT230.12321868638165648No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGATTCAAGCGTTATCCGGATTTAT220.11786135219114968No Hit
GTGCCAGCCGCCGCGGTCACACGATTAACCCAAGTCAATAGAAGCCGGCG220.11786135219114968No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC220.11786135219114968No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC210.11250401800064287No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGCAGCCGGCG210.11250401800064287No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGC200.10714668381013608No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC200.10714668381013608No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT200.10714668381013608No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGTGCAAGCGTTATCCGGATTTAC200.10714668381013608No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCCATAGCATATGTTAAAGTTGC200.10714668381013608No Hit
GTGTCAGCAGCCGCGGTAACACGATTACCCCAAGTCAATAGAAGCCGGCG200.10714668381013608No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGC190.10178934961962928No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC190.10178934961962928No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGCAGCCGGCG190.10178934961962928No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCCATAGCGTATATTAAAGTTGC190.10178934961962928No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)