FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969382

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969382
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences48112
Sequences flagged as poor quality0
Sequence length239
%GC51

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC46159.592201529763884No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC45839.525690056534753No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT45639.484120385766545No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT42758.885517126704357No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC39708.251579647489192No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT37457.783920851346858No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC35377.3515962753575No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT34397.147904888593281No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT4871.0122214832058531No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT4160.8646491519787164No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT3280.6817426005986033No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG3250.6755071499833721No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGT3160.6568007981376788No Hit
GTGTCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT3010.6256235450615232No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT2970.6173096109078816No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT2960.6152311273694713No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT2760.5736614566012637No Hit
GTGTCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT2760.5736614566012637No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGT2740.569504489524443No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG2590.5383272364482873No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGT2580.536248752909877No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG2470.5133854339873628No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG2420.5029930162953109No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGT2330.48428666444961754No Hit
GTGCCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT2320.4822081809112072No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT2290.4759727302959761No Hit
GTGCCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT2230.46350182906551385No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT2180.45310941137346195No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCCATAGCGTATATTAAAGTTGT1740.3616561356834054No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCCATAGCGTATATTAAAGTTGT1670.34710675091453275No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCCATAGCGTATATTAAAGTTGT1560.3242434319920186No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCACTAGCGTATATTAAAGTTGT1410.29306617891586295No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCCATAGCGTATATTAAAGTTGT1330.27643831060858No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCACTAGCGTATATTAAAGTTGT1330.27643831060858No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCACTAGCGTATATTAAAGTTGT1170.243182573994014No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCACTAGCGTATATTAAAGTTGT1000.20784835384103756No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTCTATTAAAGTTGT990.2057698703026272No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTCTATTAAAGTTGT950.1974559361489857No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTCTATTAAAGTTGT860.17874958430329232No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC860.17874958430329232No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTCTATTAAAGTTGT850.17667110076488193No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAT800.16627868307283006No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC730.15172929830395743No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAT710.14757233122713667No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAT660.1371799135350848No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC630.13094446291985368No Hit
GTGTCAGCAGCCGCGGTAATTCCCGCTCCAATAGCGTATATTAAAGTTGT610.12678749584303292No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAT580.1205520452278018No Hit
GTGTCAGCCGCCGCGGTAATTCCCGCTCCAATAGCGTATATTAAAGTTGT520.10808114399733955No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAT520.10808114399733955No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAT510.10600266045892916No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)