FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969389

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969389
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences68448
Sequences flagged as poor quality0
Sequence length239
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA36255.2959911173445535No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA30004.38288920056101No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA29994.3814282374941556No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA28184.116993922393642No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA26503.8715521271622255No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA26023.8014258999532493No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA25633.7444483403459556No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA25313.6976975222066386No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA25073.662634408602151No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA25063.661173445535297No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA24403.5647498831229543No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA22463.281323048153343No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA21113.084093034128097No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA20843.0446470313230485No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA20673.0198106591865357No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA19962.9160822814399254No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA18912.76268115942029No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA18572.713008415147265No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA18082.6414212248714355No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA18002.6297335203366057No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA17302.527466105656849No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA16832.4588008415147264No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA16792.4529569892473115No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA14982.1885226741467974No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA3440.5025712949976624No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA3170.46312529219261334No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2840.41491351098644225No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2750.4017648433847592No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2720.3973819541841982No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2710.3959209911173446No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2550.37254558204768584No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2540.37108461898083217No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2490.3637798036465638No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2470.3608578775128565No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2450.35793595137914913No Hit
GGACTACGGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2160.3155680224403927No Hit
GGACTACACGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2150.31410705937353905No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2090.30534128097241703No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2070.3024193548387097No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2070.3024193548387097No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA2000.292192613370734No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1940.28342683496961196No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1910.27904394576905095No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1910.27904394576905095No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1870.2732000935016363No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1860.2717391304347826No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1850.2702781673679289No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1840.2688172043010753No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1800.26297335203366057No Hit
GGACTACGAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1760.25712949976624594No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1730.2527466105656849No Hit
GGACTACGCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1700.24836372136512389No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1660.2425198690977092No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1540.22498831229546515No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1490.21768349696119682No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1480.21622253389434312No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1470.21476157082748948No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1470.21476157082748948No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1470.21476157082748948No Hit
GGACTACGCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1440.21037868162692847No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA1420.20745675549322112No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1400.2045348293595138No Hit
GGACTACGGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1350.19723001402524543No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1280.18700327255726976No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1270.18554230949041609No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1160.16947171575502573No Hit
GGACTACGAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1150.16801075268817203No Hit
GGACTACGCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1130.16508882655446472No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1090.15924497428705003No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1080.15778401122019636No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1070.1563230481533427No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1050.15340112201963535No Hit

[WARN]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)