FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969396

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969396
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences119020
Sequences flagged as poor quality0
Sequence length239
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTATCCGGATTTAT93467.852461771130903No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT90047.565115106704756No Hit
GTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTATCCGGATTTAT77426.5047891110737694No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT73906.209040497395395No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT66735.606620736010755No Hit
GTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTATCCGGATTTAT56114.714333725424298No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT55254.6420769618551505No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT55224.639556377079482No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT50944.279952949084188No Hit
GTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTATCCGGATTTAT49074.122836498067552No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTTCGGGCGTTATCCGGATTTAT47353.978322970929256No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT42683.5859519408502774No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT39773.3414552176104855No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT34412.8911107376911445No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT32822.7575197445807427No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT27062.2735674676524953No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGAATTAT18461.550999831961015No Hit
GTGTCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT16701.4031255251218284No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC16401.3779196773651488No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGAATTAT15481.3006217442446648No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC14441.213241472021509No Hit
GTGTCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT14291.2006385481431692No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGAATTAT10860.9124516887917997No Hit
GTGCCAGCAGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT10670.8964879852125693No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC10110.8494370694001009No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGAATTAT9700.8149890774659722No Hit
GTGCCAGCCGCCGCGGTAATACATAGGTCGCAAGCGTTATCCGGAATTAT9180.7712989413543943No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC9010.7570156276256091No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT7440.6251050243656529No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT6230.5234414384137119No Hit
GTGTCAGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCGGATTTAT5030.42261804738699377No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT4630.38901025037808773No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT4460.3747269366493027No Hit
GTGTCAGCCGCCGCGGTAATACGTATGTCCCGAGCGTTATCCGGATTTAT4170.35036128381784576No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT4070.3419593345656192No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT3780.3175936817341623No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC3740.3142329020332717No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC3290.27642413039825237No Hit
GTGCCAGCAGCCGCGGTAATACGTATGTCCCGAGCGTTATCCGGATTTAT3020.2537388674172408No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT3010.25289867249201814No Hit
GTGTCAGCAGCCGCGGTAATACCTAGGTCGCAAGCGTTATCCGGAATTAT2630.2209712653335574No Hit
GTGCCAGCCGCCGCGGTAATACGTATGTCCCGAGCGTTATCCGGATTTAT2530.21256931608133084No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT2380.1999663922029911No Hit
GTGTCAGCCGCCGCGGTAATACCTAGGTCGCAAGCGTTATCCGGAATTAT2350.19744580742732312No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC2190.1840026886237607No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC2000.16803898504453033No Hit
GTGCCAGCAGCCGCGGTAATACCTAGGTCGCAAGCGTTATCCGGAATTAT1500.12602923878339775No Hit
GTGCCAGCCGCCGCGGTAATACCTAGGTCGCAAGCGTTATCCGGAATTAT1340.11258611997983531No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTACTCGGAATCAC1250.10502436565283145No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)