FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969402

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969402
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences80679
Sequences flagged as poor quality0
Sequence length239
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC1382617.13704929411619No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT1295716.059941248652066No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC1013912.56708685035759No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT933311.56806603948983No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC74909.283704557567644No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT73199.071753492234658No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC59207.3377210922297005No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT54786.789870970140929No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT3200.3966335725529567No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG2680.3321806170131013No Hit
GTGTCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT2030.2516144225882819No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT1850.22930378413217814No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT1830.22682482430372214No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG1720.21319054524721426No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT1660.2057536657618463No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG1510.18716146704842648No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT1490.1846825072199705No Hit
GTGTCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT1410.1747666679061466No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT1340.16609030850655065No Hit
GTGCCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT1230.15245602945004277No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT1190.1474981097931308No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG1130.14006123030776285No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT1110.13758227047930688No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC1030.12766643116548296No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT990.122708511508571No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC960.11899007176588702No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT920.11403215210897506No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)