FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969408

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969408
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences6766
Sequences flagged as poor quality0
Sequence length239
%GC51

[OK]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC5488.099320130062075No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG5397.966302098728939No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC4506.650901566656814No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG4126.089269878805793No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC3925.793674253621046No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC3525.202483003251552No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG3344.936446940585279No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG3204.729530002955956No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC1351.995270469997044No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC1341.9804906887378066No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC1221.8031333136269583No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC1011.4927579071829737No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC1001.4779781259237363No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC660.975465563109666No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC650.9606857818504286No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT630.9311262193319538No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT630.9311262193319538No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC600.8867868755542417No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC590.8720070942950044No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC540.7981081879988176No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC540.7981081879988176No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC520.768548625480343No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT510.7537688442211055No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT490.7242092817026308No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG460.6798699379249187No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC460.6798699379249187No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG460.6798699379249187No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC460.6798699379249187No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC450.6650901566656814No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT440.650310375406444No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT420.6207508128879693No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC360.532072125332545No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC330.487732781554833No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC300.44339343777712087No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG290.4286136565178835No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT290.4286136565178835No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC280.4138338752586462No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC270.3990540939994088No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG250.3694945314809341No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC250.3694945314809341No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT240.35471475022169674No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT220.325155187703222No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT220.325155187703222No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC200.29559562518474725No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC190.2808158439255099No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT170.25125628140703515No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC170.25125628140703515No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT170.25125628140703515No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGGGCTAGCGTTGTTCGGAATTAC160.23647650014779784No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC150.22169671888856043No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTTAT150.22169671888856043No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTCAC130.19213715637008574No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT130.19213715637008574No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGCAGCCGGCG130.19213715637008574No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTTAT130.19213715637008574No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC130.19213715637008574No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT120.17735737511084837No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGGGCTAGCGTTGTTCGGAATTAC120.17735737511084837No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT120.17735737511084837No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC120.17735737511084837No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT120.17735737511084837No Hit
GTGTCAGCCGCCGCGGTAACACGATTCACCCAAGTCAATAGAAGCCGGCG120.17735737511084837No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGCAGCCGGCG120.17735737511084837No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC110.162577593851611No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT110.162577593851611No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTTAT110.162577593851611No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT110.162577593851611No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTTAT110.162577593851611No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT100.14779781259237362No Hit
GTGTCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT100.14779781259237362No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCACTAGAAGCCGGCG100.14779781259237362No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC100.14779781259237362No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCACTAGAAGCCGGCG100.14779781259237362No Hit
GTGTCAGCAGCCGCGGTAACACGATTCACCCAAGTCAATAGAAGCCGGCG100.14779781259237362No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT100.14779781259237362No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC90.13301803133313625No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT90.13301803133313625No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGC90.13301803133313625No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT90.13301803133313625No Hit
GTGCCAGCCGCCGCGGTAACACGATTCACCCAAGTCAATAGAAGCCGGCG90.13301803133313625No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT90.13301803133313625No Hit
GTGTCAGCCGCCGCGGTAACACGATTACCCCAAGTCAATAGAAGCCGGCG80.11823825007389892No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC80.11823825007389892No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGCAGCCGGCG80.11823825007389892No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC80.11823825007389892No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGC80.11823825007389892No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC80.11823825007389892No Hit
GTGTCAGCAGCCGCGGTAACACGATTACCCCAAGTCAATAGAAGCCGGCG70.10345846881466154No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCACTAGAAGCCGGCG70.10345846881466154No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTATCCGGATTCAC70.10345846881466154No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGCAGCCGGCG70.10345846881466154No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)