FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969426

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969426
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences55697
Sequences flagged as poor quality0
Sequence length239
%GC53

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC1227322.03529813095858No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC909616.331220712067076No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC716712.867838483221716No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC577410.366806111639765No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT25454.569366393163007No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT19193.4454279404635795No Hit
GTGTCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT15402.764960410794118No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT14762.6500529651507265No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT12412.2281271881788967No Hit
GTGTCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT11912.1383557462699967No Hit
GTGCCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT9601.7236116846508789No Hit
GTGCCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT7381.3250264825753633No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT3910.7020126757275975No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC3450.6194229491714096No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT2990.5368332226152217No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT2950.5296515072625096No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC2720.48835664398441564No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC2640.4739932132789917No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC2310.41474406161911775No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT2300.41294863278093974No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT2140.3842217713700918No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC2060.3698583406646677No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT1900.34113147925381976No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT1760.31599547551932783No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC1710.30701833132843775No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC1650.2962457582993698No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT1420.2549508950212758No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC1280.22981489128678384No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT1150.20647431639046987No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT870.1562023089214859No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAT790.14183887821606192No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAT730.13106630518699391No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT660.11849830331974792No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAT640.11490744564339192No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGCGCGAGCGTTGTCCGGAATTAT610.10952115912885793No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT560.10054401493796794No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)