FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969432

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969432
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences7643
Sequences flagged as poor quality0
Sequence length239
%GC55

[WARN]Per base sequence quality

Per base quality graph

[WARN]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC5647.379301321470627No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC5206.803611147455188No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC4355.69148240219809No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC3724.867198743948712No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC3534.618605259714772No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC3023.951328012560513No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT2883.768153866282873No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC2513.2840507654062536No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC2282.9831218108072743No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT2072.708360591390815No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT1521.9887478738715165No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT1201.570064110951197No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC831.085961010074578No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC821.0728771424833181No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC750.9812900693444984No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC700.9158707313881983No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC700.9158707313881983No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT680.8897029962056784No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTCCGAGCGTTGTCCGGAATTAT560.7326965851105587No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC560.7326965851105587No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC550.7196127175192987No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC500.6541933795629988No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC480.6280256443804788No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC480.6280256443804788No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTCCGAGCGTTGTCCGGAATTAT470.6149417767892189No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC470.6149417767892189No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT440.575690174015439No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGC430.562606306424179No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTGTCCGGAATTAC420.549522438832919No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC410.5364385712416591No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGC400.523354703650399No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC380.49718696846787913No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC380.49718696846787913No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT380.49718696846787913No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC380.49718696846787913No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGC330.43176763051157924No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT320.4186837629203193No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGC310.4055998953290593No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC310.4055998953290593No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGC300.39251602773779926No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT300.39251602773779926No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC290.37943216014653935No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTGTCCGGAATTAC290.37943216014653935No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGC290.37943216014653935No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTGTCCGGAATTAC270.3532644249640194No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTGTCCGGAATTAC270.3532644249640194No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTATTCGGAATTAC270.3532644249640194No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTCCGAGCGTTGTCCGGAATTAT270.3532644249640194No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC270.3532644249640194No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC270.3532644249640194No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT260.34018055737275943No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC260.34018055737275943No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTCCGAGCGTTGTCCGGAATTAT250.3270966897814994No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC240.3140128221902394No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCCATAGCGTATATTAAAGTTGC240.3140128221902394No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCCATAGCGTATATTAAAGTTGC220.2878450870077195No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC210.2747612194164595No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCCATAGCGTATATTAAAGTTGC210.2747612194164595No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTATTCGGAATTAC200.2616773518251995No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC200.2616773518251995No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTATTCGGAATTAC190.24859348423393957No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCCATAGCATATGTTAAAGTTGC190.24859348423393957No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT190.24859348423393957No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC190.24859348423393957No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCACTAGCGTATATTAAAGTTGC190.24859348423393957No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT170.2224257490514196No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC170.2224257490514196No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGC170.2224257490514196No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC170.2224257490514196No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGC160.20934188146015964No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTAC160.20934188146015964No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC150.19625801386889963No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTATTCGGAATTAC150.19625801386889963No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT150.19625801386889963No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC150.19625801386889963No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTCTATTAAAGTTGC140.18317414627763967No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCCATAGCATATGTTAAAGTTGC140.18317414627763967No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC140.18317414627763967No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC140.18317414627763967No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT140.18317414627763967No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT140.18317414627763967No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAT140.18317414627763967No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC130.17009027868637971No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCCATAGCGTATATTAAAGTTGC130.17009027868637971No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC130.17009027868637971No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC120.1570064110951197No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTAC120.1570064110951197No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT120.1570064110951197No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTCCAAGCGTTAATCGGAATTAC120.1570064110951197No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTAC110.14392254350385975No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATGAC110.14392254350385975No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGATCCAAGCGTTGTCCGGATTTAT110.14392254350385975No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAT110.14392254350385975No Hit
GTGTCAGCAGCCGCGGTAATACAGAGACTGCAAGCGTTATTCGGATTCAC110.14392254350385975No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGATCCAAGCGTTGTCCGGATTTAT110.14392254350385975No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGGGCTAGCGTTGTTCGGAATTAC110.14392254350385975No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAT110.14392254350385975No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTATCCGGAATTAT110.14392254350385975No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCGAGCGTTGTCCGGAATGAT110.14392254350385975No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT100.13083867591259976No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGGGCTAGCGTTGTTCGGAATTAC100.13083867591259976No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTAATCGGAATTAC100.13083867591259976No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT90.11775480832133978No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGATGCAAGCGTTATCCGGAATGAT90.11775480832133978No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGATCCAAGCGTTGTCCGGATTTAT90.11775480832133978No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATTAC90.11775480832133978No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCCATAGCATATGTTAAAGTTGC90.11775480832133978No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTCTATTAAAGTTGC90.11775480832133978No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGATTTAC80.10467094073007982No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCACTAGCGTATATTAAAGTTGC80.10467094073007982No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAT80.10467094073007982No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC80.10467094073007982No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATCAC80.10467094073007982No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT80.10467094073007982No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTCTATTAAAGTTGC80.10467094073007982No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTATCCGGAATTAT80.10467094073007982No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT80.10467094073007982No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCACTAGCGTATATTAAAGTTGC80.10467094073007982No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)