FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969434

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969434
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences9358
Sequences flagged as poor quality0
Sequence length239
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC4765.086556956614661No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC4184.466766403077581No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC3974.242359478521052No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC3333.5584526608249623No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC3263.483650352639453No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC2742.9279760632613807No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT2702.885231887155375No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC2592.76768540286386No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC2382.5432784783073306No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTAATCGGAATTAC2292.447104082068818No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC1992.1265227612737765No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC1952.083778585167771No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT1932.062406497114768No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC1611.7204530882667237No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT1541.6456507800812141No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGAATTAC1481.5815345159222056No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT1391.4853601196836932No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC1371.4639880316306904No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTCCGAGCGTTGTCCGGAATTAT1341.4319298995511862No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTCCGAGCGTTGTCCGGAATTAT1311.399871767471682No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT1301.3891857234451805No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC1191.2716392391536653No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAT1181.260953195127164No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC1001.068604402650139No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC991.0579183586236376No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTCCGAGCGTTGTCCGGAATTAT991.0579183586236376No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT971.0365462705706348No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTCCGAGCGTTGTCCGGAATTAT941.0044881384911306No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC910.9724300064116265No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAT910.9724300064116265No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC910.9724300064116265No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT870.9296858303056208No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC850.9083137422526181No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT750.8014533019876042No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAT730.7800812139346014No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCGAGCGTTAATCGGATTTAC660.7052789057490917No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT600.6411626415900834No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCAAGCGTTAATCGGAATTAC600.6411626415900834No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC580.6197905535370806No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGATTTAC550.5877324214575764No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGATTTAC540.577046377431075No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC530.5663603334045736No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCGAGCGTTGTCCGGATTTAT510.5449882453515709No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT510.5449882453515709No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAACCGGCG500.5343022013250694No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAC500.5343022013250694No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAACCGGCG470.5022440692455653No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT460.4915580252190639No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATCAC380.4060696730070528No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC380.4060696730070528No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAACCGGCG380.4060696730070528No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT370.3953836289805514No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC370.3953836289805514No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC370.3953836289805514No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT370.3953836289805514No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGATTTAC360.38469758495405004No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGATGCAAGCGTTATCCGGAATGAT330.35263945287454584No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC330.35263945287454584No Hit
GTGTCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTACTCGGAATTAC300.3205813207950417No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGTTCGGATTTAC300.3205813207950417No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC290.3098952767685403No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC270.2885231887155375No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGATGCAAGCGTTATCCGGAATGAT260.2778371446890361No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC260.2778371446890361No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC240.25646505663603336No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC240.25646505663603336No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT240.25646505663603336No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT230.24577901260953194No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATCAC230.24577901260953194No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT220.23509296858303055No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT220.23509296858303055No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC210.22440692455652916No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC210.22440692455652916No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC200.21372088053002777No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC200.21372088053002777No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT200.21372088053002777No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC200.21372088053002777No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAACCGGCG180.19234879247702502No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATCAC180.19234879247702502No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGC170.18166274845052363No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGATGCAAGCGTTATCCGGAATGAT170.18166274845052363No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGTGCAAGCGTTAATCGGAATTAC170.18166274845052363No Hit
GTGCCAGCAGCCGCGGTCACACGATTAACCCAAGTCAATAGAAACCGGCG170.18166274845052363No Hit
GTGTCAGCAGCCGCGGTCACACGATTAACCCAAGTCAATAGAAACCGGCG170.18166274845052363No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT170.18166274845052363No Hit
GTGTCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT160.1709767044240222No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC160.1709767044240222No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGGGCTAGCGTTGCTCGGAATCAC160.1709767044240222No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAT160.1709767044240222No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGATGCAAGCGTTATCCGGAATGAT150.16029066039752085No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAT150.16029066039752085No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT150.16029066039752085No Hit
GTGTCAGCCGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT140.14960461637101946No Hit
GTGCCAGCAGCCGCGGTAAGTTTTAATTTTTGTAGAGAAGGAGTCTTGCT130.13891857234451804No Hit
GTGTCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTACTCGGAATTAC130.13891857234451804No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCGAGCGTTAATCGGATTTAC130.13891857234451804No Hit
GTGTCAGCAGCCGCGGTAATACGGAGGGTGCGAGCGTTGTCCGGATTTAT120.12823252831801668No Hit
GTGCCAGCCGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC120.12823252831801668No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAT120.12823252831801668No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCGAGCGTTAATCGGATTTAC110.11754648429151528No Hit
GTGCCAGCAGCCGCGGTAATACGGAGGGAGCTAGCGTTGTTCGGAATTAC110.11754648429151528No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCGAGCGTTAATCGGATTTAC110.11754648429151528No Hit
GTGTCAGCCGCCGCGGTAATACGCAGGGTGCAAGCGTTAATCGGAATTAC100.10686044026501389No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCAAGCGTTGTCCGGAATTAT100.10686044026501389No Hit
GTGCCAGCAGCCGCGGTAATACGAAGGGTGCAAGCGTTACTCGGAATTAC100.10686044026501389No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCGAGCGTTAATCGGATTTAC100.10686044026501389No Hit
GTGCCAGCCGCCGCGGTAATACGAAGGGTGCAAGCGTTACTCGGAATTAC100.10686044026501389No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)