FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969436

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969436
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59231
Sequences flagged as poor quality0
Sequence length239
%GC52

[WARN]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC701911.850213570596479No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC57839.76346845401901No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT53719.067886748493189No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT43527.3475038408941264No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC41597.02166095456771No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC34845.8820550049805No Hit
GTGTCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT33725.692964832604548No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT32945.561277033985582No Hit
GTGTCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT27794.691799902078304No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT26654.499333119481353No Hit
GTGCCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT20693.493103273623609No Hit
GTGCCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT16482.7823268221032906No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT6821.1514240853607063No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT5440.9184379801117658No Hit
GTGTCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT4920.8306461143657882No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT4180.70571153618882No Hit
GTGCCAGCAGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT3980.6719454339788287No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC3950.6668805186473299No Hit
GTGTCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTATCCGGATTTAT3950.6668805186473299No Hit
GTGCCAGCCGCCGCGGTAATACGGAAGGTCCAGGCGTTATCCGGATTTAT3460.584153568232851No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC3360.5672705171278553No Hit
GTGTCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTATCCGGATTTAT3290.5554523813543584No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT3010.5081798382603704No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGAGCGAGCGTTGTCCGGAATTAT2560.43220610828788975No Hit
GTGCCAGCAGCCGCGGTAATACGTATGTCACGAGCGTTATCCGGATTTAT2370.400128311188398No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC2220.37480373453090443No Hit
GTGCCAGCCGCCGCGGTAATACGTATGTCACGAGCGTTATCCGGATTTAT2210.3731154294204049No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTATCCGGATTTAC2050.3461025476524117No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC1440.243115935911938No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC1290.21779135925444446No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT1260.21272644392294576No Hit
GTGTCAGCAGCCGCGGTAATACGTATGGGGCGAGCGTTGTCCGGAATTAT1150.1941550877074505No Hit
GTGTCAGCAGCCGCGGTAATACGTAAGGCCCGAGCGTTGTCCGGAATCAT1110.18740186726545222No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT1080.1823369519339535No Hit
GTGTCAGCCGCCGCGGTAATACGTATGGGGCGAGCGTTGTCCGGAATTAT1020.17220712127095608No Hit
GTGTCAGCAGCCGCGGTAATACGGAAGGTCCGGGCGTTATCCGGATTTAT930.15701237527645998No Hit
GTGTCAGCCGCCGCGGTAATACGTAAGGCCCGAGCGTTGTCCGGAATCAT910.1536357650554608No Hit
GTGCCAGCCGCCGCGGTAATACGTATGGGGCGAGCGTTGTCCGGAATTAT860.14519423950296298No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC780.13168779861896643No Hit
GTGCCAGCAGCCGCGGTAATACGTATGGGGCGAGCGTTGTCCGGAATTAT770.12999949350846685No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTGTCCGGAATTAT750.1266228832874677No Hit
GTGCCAGCAGCCGCGGTAATACGTAAGGCCCGAGCGTTGTCCGGAATCAT710.11986966284546943No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGGGCTAGCGTTGTCCGGAATCAC690.1164930526244703No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT680.11480474751397073No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTATCCGGATTTAT650.10973983218247202No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)