FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969437

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969437
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences59231
Sequences flagged as poor quality0
Sequence length239
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA15222.5696003781803447No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA12782.1576539312184497No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA12302.0766152859144706No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA11621.9618105384004998No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA11191.8892134186490182No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA11161.8841485033175196No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA10851.831811044892033No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA10731.8115513835660382No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA10241.728824433151559No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA10141.7119413820465632No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA9781.651162398068579No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA9531.6089547703060898No Hit
GGACTACACGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA9301.5701237527645997No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA9181.5498640914386046No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8601.4519423950296297No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8571.4468774796981312No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8221.3877868008306462No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8071.3624622241731528No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA7981.3472674781786564No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA7931.3388259526261586No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA7931.3388259526261586No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA7761.310124765747666No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA7741.306748155526667No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA7731.3050598504161672No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA7671.29493001975317No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA7511.2679171379851768No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA7341.239215951106684No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA7321.235839340885685No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA7151.2071381540071922No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA6931.1699954415762017No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA6931.1699954415762017No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6321.0670088298357279No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6291.061943914504229No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6241.0535023889517314No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA6181.0433725582887339No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA6061.0231128969627392No Hit
GGACTACGCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA6031.0180479816312404No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA5860.9893467947527478No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA5830.9842818794212491No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA5810.9809052692002499No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA5720.9657105232057537No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA5700.9623339129847546No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA5660.9555806925427562No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA5650.9538923874322567No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA5600.945450861879759No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA5440.9184379801117658No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA5390.9099964545592679No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA5130.8661005216862792No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA5090.8593473012442808No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA5020.8475291654707839No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA4900.8272695041447891No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4720.7968800121557968No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4670.788438486603299No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA4650.7850618763822999No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA4590.7749320457193023No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4460.752984079282808No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA4280.7225945872938158No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA4080.6888284850838243No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA4070.6871401799733248No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4060.6854518748628252No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA4000.6753220441998278No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA3900.658438993094832No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA3660.6179196704428425No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3640.6145430602218432No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA3400.5740237375698536No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA3270.5520757711333593No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA3230.5453225506913609No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA3230.5453225506913609No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA3170.5351927200283635No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA3070.5183096689233678No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA2730.46090729516638246No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA2190.3697388191994057No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1280.21610305414394487No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA1250.2110381388124462No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1190.2009083081494488No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA1180.1992200030389492No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1070.18064864682345394No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA1070.18064864682345394No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA1050.1772720366024548No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1020.17220712127095608No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA1020.17220712127095608No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA990.1671422059394574No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA980.1654539008289578No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA980.1654539008289578No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCA960.16207729060795867No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA940.15870068038695953No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCA930.15701237527645998No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA930.15701237527645998No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA900.15194745994496126No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA890.15025915483446167No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA890.15025915483446167No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA860.14519423950296298No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA860.14519423950296298No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA830.1401293241714643No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA800.13506440883996557No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCA790.13337610372946598No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA750.1266228832874677No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA750.1266228832874677No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCA750.1266228832874677No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA730.12324627306646858No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCA730.12324627306646858No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA730.12324627306646858No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA710.11986966284546943No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA710.11986966284546943No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA690.1164930526244703No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA680.11480474751397073No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA670.11311644240347114No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA670.11311644240347114No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA660.11142813729297159No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA660.11142813729297159No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA660.11142813729297159No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA660.11142813729297159No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA650.10973983218247202No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA640.10805152707197244No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA640.10805152707197244No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCA640.10805152707197244No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCA630.10636322196147288No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA630.10636322196147288No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCA620.10467491685097331No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCA620.10467491685097331No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGTACCTCA610.10298661174047373No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTGCCTCA600.10129830662997416No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)