FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969439

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969439
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences66196
Sequences flagged as poor quality0
Sequence length239
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA29194.409632001933652No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA23633.5697020968034323No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA22333.3733156081938485No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA18902.855157411323947No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA18862.8491147501359597No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA17522.646685600338389No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA17302.6134509638044596No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA16932.557556347815578No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA15752.379297842769956No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA15312.3128285697020967No Hit
GGACTACACGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA15192.294700586138135No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA14252.1526980482204365No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA12401.873224968276029No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA12291.856607650009064No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA12161.8369690011481057No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA11541.7433077527343042No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA11371.7176264426853587No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA10891.6451145084295122No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA10811.633029186053538No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA9681.4623240074928998No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA9421.4230467097709831No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA9271.4003867303160313No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8391.2674481841803131No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8191.237234878240377No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA7991.2070215723004412No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA7371.1133603238866396No Hit
GGACTACGCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA7311.104296332104659No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA7131.0771043567587166No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA7051.0650190343827421No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6701.0121457489878543No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6520.9849537736419118No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6380.9638044594839567No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6050.9139525046830623No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA5640.8520152275061939No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA5550.8384192398332225No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA5490.8293552480512417No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA5470.8263339174572482No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA5390.8142485950812737No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA5240.7915886156263218No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA4890.7387153302314339No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4630.6994380325095172No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4630.6994380325095172No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4370.6601607347876005No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA4360.6586500694906037No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4320.6526074083026164No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA4310.6510967430056197No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4290.648075412411626No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4290.648075412411626No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA4220.6375007553326485No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA4140.6254154329566741No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA4010.6057767840957158No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA3960.5982234576107317No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA3720.5619674904828087No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3680.5559248292948215No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA3590.5423288416218502No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3540.5347755151368663No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3520.5317541845428726No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA3190.4819022297419783No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA3020.4562209196930328No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA2930.4426249320200616No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA2890.4365822708320744No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA2760.4169436219711161No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA2720.4109009607831289No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA2670.4033476342981449No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA2610.3942836425161641No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA2550.38521965073418335No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA2160.3263037041513082No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA2070.31270771647833706No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA2010.30364372469635625No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA1900.28702640642939153No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA1650.24925977400447158No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1620.24472777811348118No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGTACCTCA1610.24321711281648437No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCA1370.20696114568856125No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1110.1676838479666445No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1060.16013052148166051No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA1030.15559852559067014No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA920.13898120732370536No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA920.13898120732370536No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA900.13595987672971177No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA830.12538521965073418No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGATACCCGCACTTTCGAGCTTCA830.12538521965073418No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCA820.12387455435373738No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCA810.12236388905674059No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCA810.12236388905674059No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCA790.11934255846274701No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA760.1148105625717566No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA720.1087679013837694No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCA690.10423590549277903No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA680.10272524019578222No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATACCCGCACCTTCGAGCTTAA680.10272524019578222No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGCGCTTCA680.10272524019578222No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)