FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969441

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969441
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences49538
Sequences flagged as poor quality0
Sequence length239
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA14132.8523557672897573No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA12712.5657071339173965No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA12352.4930356494004604No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA10912.2023497113327144No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA9731.964148734304978No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA9441.9056078162218901No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA9071.830917679357261No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA9031.8228430699664904No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA8901.7966005894464856No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8791.774395413621866No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA8621.7400783237110904No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8401.6956679720618515No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA8001.6149218781541443No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA7721.5583996124187494No Hit
GGACTACACGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA7541.522063870160281No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA7331.4796721708587348No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA7251.4635229520771933No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA7141.4413177762525737No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA7071.427187209818725No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA6761.364608987040252No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6371.2858815454802375No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA6261.263676369655618No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA6211.2535831079171544No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA6201.2515644555694618No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA6111.2333965844402277No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA5981.2071541039202227No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA5741.1587064475755986No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA5721.1546691428802132No Hit
GGACTACCCGGGTTTCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA5711.1526504905325206No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA5671.1445758811417497No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA5571.124389357664823No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA5311.0719043966248132No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA5301.0698857442771206No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA5251.059792482538657No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA5251.059792482538657No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA5231.0557551778432717No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4800.9689531268924866No Hit
GGACTACACGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA4790.966934474544794No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA4780.9649158221971013No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA4700.9487666034155597No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4670.9427106463724817No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA4640.9366546893294038No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA4500.908393556461706No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA4280.8639832048124673No Hit
GGACTACCGGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA4130.833703419597077No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4120.8316847672493842No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA4100.827647462553999No Hit
GGACTACGCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA4090.8256288102063063No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA4090.8256288102063063No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3960.7993863296863014No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3910.789293067947838No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA3820.7711251968186039No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3730.7529573256893698No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA3650.7368081069078283No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA3640.7347894545601357No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3490.7045096693447455No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA3490.7045096693447455No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTACCCATGCTTTCGCTCCTCA3440.694416407606282No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3400.6863417982155113No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3320.6701925794339699No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA3310.6681739270862772No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA3180.6419314465662723No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA2910.58742783317857No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA2850.575315919092414No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA2790.5632040050062578No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA2710.5470547862247164No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA2670.5389801768339457No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA2570.5187936533570189No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA2390.48245791109855063No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA2340.47236464936008715No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTACCCACGCTTTCGAACCTCA2020.40776777423392147No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA1910.38556259840930196No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1730.3492268561508337No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1260.2543501958092777No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1230.2482942387661997No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1100.22205175824619483No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1090.22003310589850217No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1090.22003310589850217No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA1000.20186523476926804No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA960.1937906253784973No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA830.16754814485849248No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA820.1655294925107998No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA800.16149218781541444No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA790.15947353546772175No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA790.15947353546772175No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA790.15947353546772175No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA680.13726835964310227No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA660.1332310549477169No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA630.12717509790463885No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA620.1251564455569462No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA590.11910048851386813No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA560.11304453147079009No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA550.11102587912309742No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA540.10900722677540473No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA520.10496992208001937No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA520.10496992208001937No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGAGCCTCA510.10295126973232671No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA510.10295126973232671No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)