FastQCFastQC Report
Tue 7 May 2024
EGAF00004969454

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969454
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences12853
Total Bases3 Mbp
Sequences flagged as poor quality0
Sequence length239
%GC52

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT375229.19162841359994No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT292122.726211779351125No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT259720.205399517622343No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT210116.346378277444952No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGCATTAT540.4201353769547966No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGCATTAT510.3967945226795301No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGCATTAT460.35789309888741927No Hit
GTGTCAGCAGCCGCGGTCATACGTAGGTGGCAAGCGTTATCCGGAATTAT300.23340854275266473No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGCATTAT270.2100676884773983No Hit
GTGTCAGCCGCCGCGGTCATACGTAGGTGGCAAGCGTTATCCGGAATTAT190.147825410410021No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGACTTAT190.147825410410021No Hit
GTGTCAGCAGCCGCGGTAATCCGTAGGTGGCAAGCGTTATCCGGAATTAT160.12448455613475454No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGGGCAAGCGTTATCCGGAATTAT150.11670427137633237No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGACTTAT150.11670427137633237No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGACTTAT140.10892398661791021No Hit
GTGTCAGCAGCCGCGGTACTACGTAGGTGGCAAGCGTTATCCGGAATTAT130.10114370185948807No Hit
GTGCCAGCCGCCGCGGTCATACGTAGGTGGCAAGCGTTATCCGGAATTAT130.10114370185948807No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.12.1)