FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969462

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969462
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences63495
Sequences flagged as poor quality0
Sequence length239
%GC52

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT1165518.355776045357903No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT925614.577525789432238No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT678210.68115599653516No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC636510.024411370974093No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT55138.682573430978817No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC49627.814788566028821No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC36185.698086463501063No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTGTCCGGAATTAC29574.657059610992992No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT13422.1135522482085203No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT9881.5560280337034411No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT7531.1859201511930073No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT5940.9355067328136074No Hit
GTGTCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT4090.6441452082841168No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC3550.559099141664698No Hit
GTGTCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT2890.45515394912985274No Hit
GTGCCAGCAGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT2620.4126309158201434No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC2560.4031813528624301No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC2150.33860933931805653No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAT2110.3323096306795811No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAT1780.28033703441215846No Hit
GTGCCAGCCGCCGCGGTAATACGTAAGGACCGAGCGTTGTCCGGAATCAT1730.2724623986140641No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC1720.27088747145444525No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT1270.2000157492715962No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT1050.1653673517599811No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAT1030.16221749744074337No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGGTGCGAGCGTTAATCGGAATTAT980.15434286164264902No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT920.14489329868493583No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGT790.12441924560989055No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTACAGTTGT770.12126939129065281No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTCAAGTTGT700.11024490117332074No Hit

[OK]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)