FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969468

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969468
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32151
Sequences flagged as poor quality0
Sequence length239
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GTGTCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT732722.78933781219869No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT533016.578022456533233No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT402712.525271375695935No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTGGCAAGCGTTATCCGGAATTAT30019.334079810892352No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG16615.1662467730397195No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG11923.7075052097912975No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG8942.780628907343473No Hit
GTGTCAGCAGCCGCGGTAGTCCGTACAAGAGTGAAGATCGGAAGAGCGTC6251.9439519766103697No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGAAGCCGGCG6231.9377313302852168No Hit
GTGTCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC5151.6018164287269445No Hit
GTGTCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT4331.346769929395664No Hit
GTGTCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC3891.2099157102422942No Hit
GTGTCAGAAACCCCGGTAGTCCGTACAAGAGTGAAGATCGGAAGAGCGTC3130.9735311498864733No Hit
GTGTCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT2920.9082143634723647No Hit
GTGCCAGCAGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC2650.8242356380827968No Hit
GTGCCAGCCGCCGCGGTAATACAGAGGGTGCAAGCGTTAATCGGAATTAC2330.7247052968803459No Hit
GTGCCAGCAGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT2240.6967123884171565No Hit
GTGCCAGCCGCCGCGGTAATACGTAGGTCCCGAGCGTTGTCCGGATTTAT1790.5567478461012099No Hit
GTGTCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT1650.5132033218251376No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG1410.43855556592329936No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG1090.3390252247208485No Hit
GTGTCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT1030.32036328574538897No Hit
GTGCCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG810.25193617616870395No Hit
GTGCCAGCAGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT790.24571552984355075No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGACGCCGGCG770.23949488351839754No Hit
GTGTCAGCCGCCGCGGTAGTCCGTACAAGAGTGAAGATCGGAAGAGCGTC720.2239432677055146No Hit
GTGTCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC590.1835090665920189No Hit
GTGCCAGCCGCCGCGGTAATTCCAGCTCCAATAGCGTATATTAAAGTTGT590.1835090665920189No Hit
GTGTCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGCAGCCGGCG530.16484712761655934No Hit
GTGTCAGCCGCCGCGGTAACACGATTAACCCAAGTCAATAGCAGCCGGCG420.13063357282821686No Hit
GTGTCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC410.12752324966564027No Hit
GTGCCAGCAGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC410.12752324966564027No Hit
GTGCCAGCCGCCGCGGTAATACCGAGGGTGCAAGCGTTAATCGGAATTAC340.10575098752760412No Hit
GTGCCAGCAGCCGCGGTAACACGATTAACCCAAGTCAATAGCAGCCGGCG340.10575098752760412No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)