FastQCFastQC Report
Thu 1 Sep 2022
EGAF00004969469

Summary

[OK]Basic Statistics

MeasureValue
FilenameEGAF00004969469
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences32151
Sequences flagged as poor quality0
Sequence length239
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per tile sequence quality

Per tile quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GGACTACTAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA12643.931448477496812No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA11483.5706509906379273No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA10683.3218251376318No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA10313.206743180616466No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA9803.04811669932506No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA9683.010792821374141No Hit
GGACTACTAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA9612.9890205592361045No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA9222.8677179558956176No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA9132.8397250474324283No Hit
GGACTACACGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8672.6966501819539053No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8142.5318030543373458No Hit
GGACTACTGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA8022.4944791763864265No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA7852.4416036826226244No Hit
GGACTACAAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA7842.4384933594600477No Hit
GGACTACTCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA7612.3669559267207863No Hit
GGACTACCAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA7182.2332120307299927No Hit
GGACTACAGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6912.1492333053404247No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6852.1305713663649652No Hit
GGACTACACGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6722.0901371652514698No Hit
GGACTACGAGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6622.0590339336257037No Hit
GGACTACGGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6602.0528132873005505No Hit
GGACTACCGCGGCTGCTGACACGTACTCTAGACTAGATCGGAAGAGCACA6552.0372616714876677No Hit
GGACTACCGGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA6031.8755248670336848No Hit
GGACTACGCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA5971.8568629280582252No Hit
GGACTACCCGGGTATCTAATCCTGTTTGATCCCCACGCTTTCGCACATCA5151.6018164287269445No Hit
GGACTACCGGGGTTTCTGACACGTACTCTAGACTAGATCGGAAGAGCACA3231.0046343815122392No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2700.8397872538956799No Hit
GGACTACTCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2550.7931324064570309No Hit
GGACTACAAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2510.7806911138067245No Hit
GGACTACTGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2450.762029174831265No Hit
GGACTACGAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2310.7184846505551926No Hit
GGACTACTAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2220.6904917420920034No Hit
GGACTACCAGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC2120.6593885104662374No Hit
GGACTACTCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1860.578520108239246No Hit
GGACTACAGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1850.5754097850766695No Hit
GGACTACACGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1840.5722994619140929No Hit
GGACTACCGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1820.5660788155889397No Hit
GGACTACGGGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1780.5536375229386333No Hit
GGACTACAAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1730.5380859071257503No Hit
GGACTACCCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1660.5163136449877143No Hit
GGACTACTGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1640.5100929986625611No Hit
GGACTACGAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1640.5100929986625611No Hit
GGACTACGCGGGTTTCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1640.5100929986625611No Hit
GGACTACCAGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1600.49765170601225467No Hit
GGACTACAGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1470.45721750489875895No Hit
GGACTACACGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1410.43855556592329936No Hit
GGACTACGGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1350.41989362694783994No Hit
GGACTACGCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1330.4136729806226867No Hit
GGACTACCGGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1210.37634910267176763No Hit
GGACTACCCGGGTATCTAATCCCAGTTTGGGTCTTAGCTATTGTGTGTTC1070.3328045783956953No Hit
GGACTACTGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1040.3234736089079655No Hit
GGACTACAAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1040.3234736089079655No Hit
GGACTACTAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA1010.31414263942023574No Hit
GGACTACTCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA910.28303940779446984No Hit
GGACTACGGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA890.27681876146931667No Hit
GGACTACGAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA880.2737084383067401No Hit
GGACTACCAGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA860.2674877919815869No Hit
GGACTACAGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA780.24260520668097416No Hit
GGACTACCGCGGCGGCTGACACGTACTCTAGACTAGATCGGAAGAGCACA760.23638456035582098No Hit
GGACTACTAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA750.23327423719324436No Hit
GGACTACTAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA750.23327423719324436No Hit
GGACTACGCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA710.22083294454293803No Hit
GGACTACACGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA700.21772262138036141No Hit
GGACTACTGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA680.21150197505520824No Hit
GGACTACCGGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA670.20839165189263162No Hit
GGACTACCAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA640.19906068240490185No Hit
GGACTACTCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA640.19906068240490185No Hit
GGACTACAAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA620.19284003607974867No Hit
GGACTACAGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA590.1835090665920189No Hit
GGACTACGAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA590.1835090665920189No Hit
GGACTACCAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA560.17417809710428914No Hit
GGACTACTGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA560.17417809710428914No Hit
GGACTACTAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA520.16173680445398275No Hit
GGACTACTCGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA510.1586264812914062No Hit
GGACTACCCGGGTTTCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA510.1586264812914062No Hit
GGACTACGGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA500.1555161581288296No Hit
GGACTACAGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA490.15240583496625298No Hit
GGACTACAAGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA490.15240583496625298No Hit
GGACTACGCGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA480.1492955118036764No Hit
GGACTACGAGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA480.1492955118036764No Hit
GGACTACGCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA470.1461851886410998No Hit
GGACTACTGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA450.13996454231594663No Hit
GGACTACGGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA450.13996454231594663No Hit
GGACTACAAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA450.13996454231594663No Hit
GGACTACACGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA450.13996454231594663No Hit
GGACTACCGGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA430.13374389599079345No Hit
GGACTACGAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA420.13063357282821686No Hit
GGACTACTCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA420.13063357282821686No Hit
GGACTACCGGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA410.12752324966564027No Hit
GGACTACAGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA390.12130260334048708No Hit
GGACTACCCGGGTATCTAATCCTGTTTGCTCCCCACGCTTTCGCACCTCA390.12130260334048708No Hit
GGACTACCCGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA380.11819228017791049No Hit
GGACTACCAGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA380.11819228017791049No Hit
GGACTACACGGGTTTCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA360.1119716338527573No Hit
GGACTACGGGGGTATCTAATCCTGTTCGCTCCCCACGCTTTCGAGCCTCA340.10575098752760412No Hit
GGACTACTAGGGTTTCTAATCCCAGTTTGGGTCTTCGCTATTGTGTGTTC330.10264066436502753No Hit

[FAIL]Adapter Content

Adapter graph

Produced by FastQC (version 0.11.9)